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Building an antifouling zwitterionic coating on urinary catheters using an enzymatically triggered bottom-up approach

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Díaz Blanco, Carlos
Ortner, Andreas
Dimitrov, Radostin
Navarro Sentanyes, AntonioMés informacióMés informació
Mendoza Gómez, ErnestoMés informació
Tzanov, TzankoMés informacióMés informacióMés informació
Document typeArticle
Defense date2014-06-23
Rights accessRestricted access - publisher's policy
All rights reserved. This work is protected by the corresponding intellectual and industrial property rights. Without prejudice to any existing legal exemptions, reproduction, distribution, public communication or transformation of this work are prohibited without permission of the copyright holder
ProjectNOVO - Novel approaches for prevention and degeneration of pathogenic bacteria biofilms formed on medical devices e.g. catheters (EC-FP7-278402)
Abstract
Catheter associated urinary tract infections are common during hospitalization due to the formation of bacterial biofilms on the indwelling device. In this study, we report an innovative biotechnology-based approach for the covalent functionalization of silicone catheters with antifouling zwitterionic moieties to prevent biofilm formation. Our approach combines the potential bioactivity of a natural phenolics layer biocatalytically conjugated to sulfobetaine-acrylic residues in an enzymatically initiated surface radical polymerization with laccase. To ensure sufficient coating stability in urine, the silicone catheter is plasma-activated. In contrast to industrial chemical methods, the methacrylate-containing zwitterionic monomers are polymerized at pH 5 and 50 °C using as an initiator the phenoxy radicals solely generated by laccase on the phenolics-coated catheter surface. The coated catheters are characterized by X-ray photoelectron spectroscopy (XPS), Fourier transformed infrared (FTIR) analysis, atomic force microscopy (AFM), and colorimetrically. Contact angle and protein adsorption measurements, coupled with in vitro tests with the Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus in static and dynamic conditions, mimicking the operational conditions to be faced by the catheters, demonstrate reduced biofilm formation by about 80% when compared to that of unmodified urinary catheters. The zwitterionic coating did not affect the viability of the human fibroblasts (BJ-5ta) over seven days, corresponding to the extended useful life of urinary catheters.
CitationDiaz, C. [et al.]. Building an antifouling zwitterionic coating on urinary catheters using an enzymatically triggered bottom-up approach. "ACS applied materials and interfaces", 23 Juny 2014, vol. 6, núm. 14, p. 11385-11393. 
URIhttp://hdl.handle.net/2117/23639
DOI10.1021/am501961b
ISSN1944-8244
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  • GBMI - Grup de Biotecnologia Molecular i Industrial - Articles de revista [176]
  • Departament de Física i Enginyeria Nuclear (fins octubre 2015) - Articles de revista [603]
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  • Departament d'Enginyeria Química - Articles de revista [2.025]
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