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dc.contributor.authorFrancesko, Antonio
dc.contributor.authorMacedo Fernandes, Margarida Maria
dc.contributor.authorTzanov, Tzanko
dc.contributor.authorPerelshtein, Illana
dc.contributor.authorBenisvy Aharonovich, Lena
dc.contributor.authorGedanken, Aharon
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2014-07-24T08:37:53Z
dc.date.created2014-07-09
dc.date.issued2014-07-09
dc.identifier.citationFrancesko, A. [et al.]. One-step sonochemical preparation of redox-responsive nanocapsules for glutathione mediated RNA release. "Journal of materials chemistry B", 09 Juliol 2014, p. 1-27.
dc.identifier.issn2050-750X
dc.identifier.urihttp://hdl.handle.net/2117/23597
dc.description.abstractEfficient RNA delivery to targeted cells requires the use of stable interactive carriers that provide RNA protection during the extracellular transit and trigger release once internalised. One strategy to avoid the premature extracellular RNA drain coupled to sufficient intracellular release is the use of stimuli-responsive delivery materials exploiting as a triggering mechanism the redox gradient between the extra- and intracellular compartments. This work describes a facile route for preparation of redox-active nanocarriers containing disulphides that combine RNA protection and delivery on demand based on intracellular glutathione (GSH) levels. A one-step sonochemical technology was employed to generate thiolated chitosan (TC) nanocapsules with diameter between 250 - 570 nm and simultaneously load them with RNA. Their size and physiological stability were directly proportional to the extent of disulphide cross-linking, which in turn could be ruled by adjusting the processing pH and degree of chitosan thiolation. The TC processing into nanocapsules showed to be advantageous in terms of RNA condensation and protection compared to the typically employed nanocomplexation. Fluorescence microscopy imaging revealed that: i) the nanocapsules enter the human fibroblasts and migrate to the perinuclear regions within 1 h, and ii) the cargo release occurs after the internalisation. These redox-responsive and biocompatible drug carriers demonstrated an effective (~60 %) and sustained (up to 72 h) RNA release at intracellular GSH concentrations (10 mM) in vitro, based on disulphide reduction and consequent capsules disassembly.
dc.format.extent27 p.
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshMolecular biology
dc.subject.othersonochemistry
dc.subject.otherdisulphide cross - linking
dc.subject.otherredox - responsive carriers
dc.subject.otherRNA delivery
dc.subject.otherglutathione - mediated release
dc.titleOne-step sonochemical preparation of redox-responsive nanocapsules for glutathione mediated RNA release
dc.typeArticle
dc.subject.lemacBiologia molecular
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.1039/c4tb00599f
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://pubs.rsc.org/en/Content/ArticleLanding/2014/TB/C4TB00599F#!divAbstract
dc.rights.accessRestricted access - publisher's policy
drac.iddocument15013824
dc.description.versionPostprint (published version)
dc.date.lift10000-01-01
upcommons.citation.authorFrancesko, A.; Macedo, M.M.; Tzanov, T.; Perelshtein, I.; Benisvy , L.; Gedanken, A.
upcommons.citation.publishedtrue
upcommons.citation.publicationNameJournal of materials chemistry B
upcommons.citation.startingPage1
upcommons.citation.endingPage27


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Except where otherwise noted, content on this work is licensed under a Creative Commons license: Attribution-NonCommercial-NoDerivs 3.0 Spain