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dc.contributor.authorGonzalez Vazquez, Arlyng
dc.contributor.authorPlanell Estany, Josep Anton
dc.contributor.authorEngel López, Elisabeth
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.contributor.otherInstitut de Bioenginyeria de Catalunya
dc.date.accessioned2014-07-01T10:22:25Z
dc.date.created2014-06-01
dc.date.issued2014-06-01
dc.identifier.citationGonzalez, A.; Planell, J.; Engel, E. Extracellular calcium and CaSR drive osteoinduction in mesenchymal stromal cells. "Acta biomaterialia", 01 Juny 2014, vol. 10, núm. 6, p. 2824-2833.
dc.identifier.issn1742-7061
dc.identifier.urihttp://hdl.handle.net/2117/23357
dc.description.abstractBone is the main store of calcium and progenitor cells in the body. During the resorption process, the local calcium concentration reaches 8-40 mM, and the surrounding cells are exposed to these fluctuations in calcium. This stimulus is a signal that is detected through the calcium sensing receptor (CaSR), which modulates chemotactic and proliferative G protein-dependent signaling pathways. The objective of the present work is to evaluate the roles of extracellular calcium ([Ca2+](o)) and the CaSR in osteoinduction. Rat bone marrow mesenchymal stromal cells (rBMSCs) were stimulated with 10 mM of Ca2+. Several experiments were conducted to demonstrate the effect of [Ca2+](o) on chemotaxis, proliferation and differentiation on the osteoblastic lineage. It was found that [Ca2+](o) induces rBMSCs to migrate and proliferate in a concentration-dependent manner. Real-time polymerase chain reaction and immunofluorescence also revealed that 10 mM Ca2+ stimulates overexpression of osteogenic markers in rBMSCs, including alkaline phosphatase (ALP), bone sialoprotein, collagen Ia1 and osteocalcin. Functional assays determining ALP activity and mineralization tests both corroborate the increased expression of these markers in rBMSCs stimulated with Ca2+. Moreover, CaSR blockage inhibited the cellular response to stimulation with high concentrations of [Ca2+](o), revealing that the CaSR is a key modulator of these cellular responses. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
dc.format.extent10 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshRegenerative medicine
dc.subject.lcshCalcium--Receptors
dc.subject.otherCalcium sensing receptor (CaSR)
dc.subject.otherExtracellular calcium
dc.subject.otherMesenchymal stromal cells (MSCs)
dc.subject.otherOsteoinduction
dc.subject.otherRegenerative medicine
dc.subject.otherSENSING-RECEPTOR
dc.subject.otherBONE-MARROW
dc.subject.otherSTEM-CELLS
dc.subject.otherIN-VITRO
dc.subject.otherOSTEOBLAST FUNCTION
dc.subject.otherMC3T3-E1 CELLS
dc.subject.otherDIFFERENTIATION
dc.subject.otherPROLIFERATION
dc.subject.otherEXPRESSION
dc.subject.otherTISSUE
dc.titleExtracellular calcium and CaSR drive osteoinduction in mesenchymal stromal cells
dc.typeArticle
dc.subject.lemacMedicina regenerativa
dc.subject.lemacCalci
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1016/j.actbio.2014.02.004
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1742706114000567#
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac14957118
dc.description.versionPostprint (published version)
dc.date.lift10000-01-01
local.citation.authorGonzalez, A.; Planell, J.; Engel, E.
local.citation.publicationNameActa biomaterialia
local.citation.volume10
local.citation.number6
local.citation.startingPage2824
local.citation.endingPage2833


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Attribution-NonCommercial-NoDerivs 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain