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dc.contributor.authorMas Moruno, Carlos
dc.contributor.authorDorfner, Petra M.
dc.contributor.authorManzenrieder, Florian
dc.contributor.authorNeubauer, Stefanie
dc.contributor.authorReuning, Ute
dc.contributor.authorBurgkart, Rainer
dc.contributor.authorKessler, Horst
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.date.accessioned2014-04-30T09:50:33Z
dc.date.created2013
dc.date.issued2013
dc.identifier.citationMas-Moruno, C. [et al.]. Behavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin avß3-selective cyclic RGD peptides. "Journal of biomedical materials research. Part A", 2013, vol. 101, núm. 1, p. 87-97.
dc.identifier.issn1549-3296
dc.identifier.urihttp://hdl.handle.net/2117/22773
dc.description.abstractIt is well known that functionalization of surfaces with cell adhesive peptides mimicking the integrin binding motif of extracellular matrix proteins is a feasible approach to improve osseointegration of implant materials. Also, modi- fication of the surface properties of the material (e.g., rough- ness) strongly influences cell behavior. However, these two approaches are rarely studied together. This study addressed the hypothesis that the combination of peptide functionaliza- tion and surface roughness will have an enhancing effect on the adhesion process of osteoblasts. To test this hypothesis, a series of a v b 3-selective cyclic RGD peptides were prepared and immobilized on trimmed ( S a ¼ 0.74 l m, smooth) and sandblasted ( S a ¼ 3.24 l m, rough) Ti6Al4V disks. Effects of these surface modifications were evaluated with respect to integrin a v b 3-mediated adhesive capacity, cell morphology, and spreading of primary human osteoblasts. After 3 h of incubation, osteoblasts adhered more strongly on sand- blasted than on trimmed noncoated Ti6Al4V surfaces. Their attachment efficiency was further enhanced in the presence of RGD peptides. However, peptide functionalization had a relatively stronger impact on osteoblast attachment on trimmed surfaces compared with sandblasted surfaces. Cell morphology after 3 h of culture was exclusively altered by surface topography. RGD coating was critical for osteoblast spreading on both trimmed and sandblasted materials after 1 h of incubation but it showed almost negligible effects after 3 h. The results of this study provide evidence that the alli- ance of RGD coating and surface topography on Ti6Al4V pos- itively influences osteoblast adhesion and spreading, especially at very early adhesion times
dc.format.extent11 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshPeptides
dc.titleBehavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin avß3-selective cyclic RGD peptides
dc.typeArticle
dc.subject.lemacPèptids
dc.identifier.doi10.1002/jbm.a.34303
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34303/abstract
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac13617704
dc.description.versionPostprint (published version)
dc.date.lift10000-01-01
local.citation.authorMas-Moruno, C.; Dorfner, P.; Manzenrieder, F.; Neubauer, S.; Reuning, U.; Burgkart, R.; Kessler, H.
local.citation.publicationNameJournal of biomedical materials research. Part A
local.citation.volume101
local.citation.number1
local.citation.startingPage87
local.citation.endingPage97


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