Mostra el registre d'ítem simple
Behavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin avß3-selective cyclic RGD peptides
dc.contributor.author | Mas Moruno, Carlos |
dc.contributor.author | Dorfner, Petra M. |
dc.contributor.author | Manzenrieder, Florian |
dc.contributor.author | Neubauer, Stefanie |
dc.contributor.author | Reuning, Ute |
dc.contributor.author | Burgkart, Rainer |
dc.contributor.author | Kessler, Horst |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica |
dc.date.accessioned | 2014-04-30T09:50:33Z |
dc.date.created | 2013 |
dc.date.issued | 2013 |
dc.identifier.citation | Mas-Moruno, C. [et al.]. Behavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin avß3-selective cyclic RGD peptides. "Journal of biomedical materials research. Part A", 2013, vol. 101, núm. 1, p. 87-97. |
dc.identifier.issn | 1549-3296 |
dc.identifier.uri | http://hdl.handle.net/2117/22773 |
dc.description.abstract | It is well known that functionalization of surfaces with cell adhesive peptides mimicking the integrin binding motif of extracellular matrix proteins is a feasible approach to improve osseointegration of implant materials. Also, modi- fication of the surface properties of the material (e.g., rough- ness) strongly influences cell behavior. However, these two approaches are rarely studied together. This study addressed the hypothesis that the combination of peptide functionaliza- tion and surface roughness will have an enhancing effect on the adhesion process of osteoblasts. To test this hypothesis, a series of a v b 3-selective cyclic RGD peptides were prepared and immobilized on trimmed ( S a ¼ 0.74 l m, smooth) and sandblasted ( S a ¼ 3.24 l m, rough) Ti6Al4V disks. Effects of these surface modifications were evaluated with respect to integrin a v b 3-mediated adhesive capacity, cell morphology, and spreading of primary human osteoblasts. After 3 h of incubation, osteoblasts adhered more strongly on sand- blasted than on trimmed noncoated Ti6Al4V surfaces. Their attachment efficiency was further enhanced in the presence of RGD peptides. However, peptide functionalization had a relatively stronger impact on osteoblast attachment on trimmed surfaces compared with sandblasted surfaces. Cell morphology after 3 h of culture was exclusively altered by surface topography. RGD coating was critical for osteoblast spreading on both trimmed and sandblasted materials after 1 h of incubation but it showed almost negligible effects after 3 h. The results of this study provide evidence that the alli- ance of RGD coating and surface topography on Ti6Al4V pos- itively influences osteoblast adhesion and spreading, especially at very early adhesion times |
dc.format.extent | 11 p. |
dc.language.iso | eng |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Enginyeria dels materials |
dc.subject.lcsh | Peptides |
dc.title | Behavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin avß3-selective cyclic RGD peptides |
dc.type | Article |
dc.subject.lemac | Pèptids |
dc.identifier.doi | 10.1002/jbm.a.34303 |
dc.description.peerreviewed | Peer Reviewed |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34303/abstract |
dc.rights.access | Restricted access - publisher's policy |
local.identifier.drac | 13617704 |
dc.description.version | Postprint (published version) |
dc.date.lift | 10000-01-01 |
local.citation.author | Mas-Moruno, C.; Dorfner, P.; Manzenrieder, F.; Neubauer, S.; Reuning, U.; Burgkart, R.; Kessler, H. |
local.citation.publicationName | Journal of biomedical materials research. Part A |
local.citation.volume | 101 |
local.citation.number | 1 |
local.citation.startingPage | 87 |
local.citation.endingPage | 97 |
Fitxers d'aquest items
Aquest ítem apareix a les col·leccions següents
-
Articles de revista [960]