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dc.contributor.authorMacedo Fernandes, Margarida Maria
dc.contributor.authorFrancesko, Antonio
dc.contributor.authorTorrent Burgués, Juan
dc.contributor.authorTzanov, Tzanko
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.identifier.citationMacedo, M.M. [et al.]. Effect of thiol-functionalisation on chitosan antibacterial activity: interaction with a bacterial membrane model. "Reactive and functional polymers", 12 Gener 2013, vol. 73, núm. 10, p. 2013-1384.
dc.description.abstractThe antibacterial activity of chitosan modified with the thiol-containing 2-iminothiolane HCl (TC-IMI) and N-acetyl- L -cysteine (TC-NAC) was studied by Langmuir film balance technique using a dipalmitoylphos- phatidylglycerol (DPPG) monolayer bacterial membrane model. The interactions of the biopolymer with the membrane model were assessed by monitoring the differences in the shape of the compression iso- therms recorded in the absence and presence of chitosan and thiolated conjugates in the subphase. A low molecularweightchitosan(15 kDa)shiftedthecompressionisothermsofDPPGmonolayerstowardslarger areas ( A 0,CS = 145 Å 2 ), confirming its membrane disturbance capacity. Further thiolation induced higher yield of expansion, more pronounced in the case of TC-IMI. The expansion of the monolayer increased sig- nificantly( A 0,TC-NAC = 150 Å 2 vs A 0,TC-IMI = 175 Å 2 )andtheelasticityatasurfacepressureof30 mN/m,typical for bio-membranes decreased to a greater extent C 1 s ; 30 TC NAC = 120 mN/m vs C 1 s ; 30 TC IMI = 87 mN/m) in presence of TC-IMI. Antibacterial tests against a Gram-negative Escherichia coli and a Gram-positive Staph- ylococcusaureus wereingoodagreementwiththesefindings,suggestingthatchitosanthiolatedwith2-imi- nothiolane HCl acts as a bactericide disrupting the integrity of the bacterial cell membrane
dc.format.extent-628 p.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshChemical engineering
dc.subject.otherThiolated chitosan
dc.subject.otherAntibacterial activity
dc.subject.otherLangmuir monolayers
dc.subject.otherCell membrane mode
dc.titleEffect of thiol-functionalisation on chitosan antibacterial activity: interaction with a bacterial membrane model
dc.subject.lemacEnginyeria química
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.rights.accessRestricted access - publisher's policy
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/228730/EU/A pilot line of antibacterial and antifungal medical textiles based on a sonochemical process/SONO
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/278402/EU/Novel approaches for prevention and degeneration of pathogenic bacteria biofilms formed on medical devices e.g. catheters/NOVO
upcommons.citation.authorMacedo, M.M.; Francesko, A.; Torrent, J.; Tzanov, T.
upcommons.citation.publicationNameReactive and functional polymers

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