Show simple item record

dc.contributor.authorBorroto Escuela, Dasiel Óscar
dc.contributor.authorRomero Fernandez, Wilber
dc.contributor.authorGarriga Solé, Pere
dc.contributor.authorCiruela, Francisco
dc.contributor.authorNarvaez, Manuel
dc.contributor.authorTarakanov, O.V.
dc.contributor.authorPalkovits, Miklós
dc.contributor.authorAgnati, Luigi F.
dc.contributor.authorFuxe, Kjell
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2014-03-10T08:04:02Z
dc.date.created2013
dc.date.issued2013
dc.identifier.citationBorroto, D. [et al.]. G protein-coupled receptor heterodimerization in the brain. "Methods in enzymology", 2013, vol. 521, p. 281-294.
dc.identifier.issn0076-6879
dc.identifier.urihttp://hdl.handle.net/2117/21956
dc.description.abstractG protein-coupled receptors (GPCRs) play critical roles in cellular processes and signaling and have been shown to form heteromers with diverge biochemical and/or pharmacological activities that are different from those of the corresponding monomers or homomers. However, despite extensive experimental results supporting the formation of GPCR heteromers in heterologous systems, the existence of such receptor heterocomplexes in the brain remains largely unknown, mostly because of the lack of appropriate methodology. Herein, we describe the in situ proximity ligation assay procedure underlining its high selectivity and sensitivity to image GPCR heteromers with confocal microscopy in brain sections. We describe here how the assay is performed and discuss advantages and disadvantages of this method compared with other available techniques.
dc.format.extent14 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Ciències de la salut::Medicina
dc.subject.lcshG proteins--Receptors
dc.subject.lcshBrain
dc.subject.lcshCell receptors
dc.subject.otherAllosteric modulation
dc.subject.otherDepression
dc.subject.otherFibroblast growth factor receptor
dc.subject.otherNeuronal plasticity
dc.subject.otherReceptor heterocomplex
dc.subject.otherSerotonin receptors
dc.titleG protein-coupled receptor heterodimerization in the brain
dc.typeArticle
dc.subject.lemacProteïnes G -- Receptors
dc.subject.lemacCervell
dc.subject.lemacReceptors cel·lulars
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.1016/B978-0-12-391862-8.00015-6
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac11758438
dc.description.versionPostprint (published version)
dc.date.lift10000-01-01
local.citation.authorBorroto, D.; Romero, W.; Garriga, P.; Ciruela, F.; Narvaez, M.; Tarakanov, O.; Palkovits, M.; Agnati, L.F.; Fuxe, K.
local.citation.publicationNameMethods in enzymology
local.citation.volume521
local.citation.startingPage281
local.citation.endingPage294


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain