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dc.contributor.authorBorroto Escuela, Dasiel Óscar
dc.contributor.authorRomero Fernandez, Wilber
dc.contributor.authorGarriga Solé, Pere
dc.contributor.authorCiruela, Francisco
dc.contributor.authorNarvaez, Manuel
dc.contributor.authorTarakanov, O.V.
dc.contributor.authorPalkovits, Miklós
dc.contributor.authorAgnati, Luigi F.
dc.contributor.authorFuxe, Kjell
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.identifier.citationBorroto, D. [et al.]. G protein-coupled receptor heterodimerization in the brain. "Methods in enzymology", 2013, vol. 521, p. 281-294.
dc.description.abstractG protein-coupled receptors (GPCRs) play critical roles in cellular processes and signaling and have been shown to form heteromers with diverge biochemical and/or pharmacological activities that are different from those of the corresponding monomers or homomers. However, despite extensive experimental results supporting the formation of GPCR heteromers in heterologous systems, the existence of such receptor heterocomplexes in the brain remains largely unknown, mostly because of the lack of appropriate methodology. Herein, we describe the in situ proximity ligation assay procedure underlining its high selectivity and sensitivity to image GPCR heteromers with confocal microscopy in brain sections. We describe here how the assay is performed and discuss advantages and disadvantages of this method compared with other available techniques.
dc.format.extent14 p.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Ciències de la salut::Medicina
dc.subject.lcshG proteins--Receptors
dc.subject.lcshCell receptors
dc.subject.otherAllosteric modulation
dc.subject.otherFibroblast growth factor receptor
dc.subject.otherNeuronal plasticity
dc.subject.otherReceptor heterocomplex
dc.subject.otherSerotonin receptors
dc.titleG protein-coupled receptor heterodimerization in the brain
dc.subject.lemacProteïnes G -- Receptors
dc.subject.lemacReceptors cel·lulars
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.rights.accessRestricted access - publisher's policy
dc.description.versionPostprint (published version)
local.citation.authorBorroto, D.; Romero, W.; Garriga, P.; Ciruela, F.; Narvaez, M.; Tarakanov, O.; Palkovits, M.; Agnati, L.F.; Fuxe, K.
local.citation.publicationNameMethods in enzymology

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