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dc.contributor.authorMendes, Ana C.
dc.contributor.authorSmith, Katherine H.
dc.contributor.authorTejeda Montes, Esther
dc.contributor.authorEngel López, Elisabeth
dc.contributor.authorReis, Rui L.
dc.contributor.authorAzevedo, Elena S.
dc.contributor.authorMata, Álvaro
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.contributor.otherInstitut de Bioenginyeria de Catalunya
dc.date.accessioned2013-12-04T10:45:45Z
dc.date.created2012-09-03
dc.date.issued2012-09-03
dc.identifier.citationMendes, A. [et al.]. Co-assembled and microfabricated bioactive membranes. "Advanced functional materials", 03 Setembre 2012, vol. 28, p. 430-438.
dc.identifier.issn1616-301X
dc.identifier.urihttp://hdl.handle.net/2117/20908
dc.description.abstractThe fabrication of hierarchical and bioactive self-supporting membranes, which integrate physical and biomolecular elements, using a single-step process that combines molecular self-assembly with soft lithography is reported. A positively charged multidomain peptide (with or without the cell-adhesive sequence arginine-glycine-aspartic acid-serine (RGDS)) self- assembles with hyaluronic acid (HA), an anionic biopolymer. Optimization of the assembling conditions enables the realization of membranes with well-controlled and easily tunable features at multiple size scales including peptide sequence, building-block co-assembly, membrane thickness, bioac- tive epitope availability, and topographical pattern morphology. Membrane structure, morphology, and bioactivity are investigated according to tempera- ture, assembly time, and variations in the experimental setup. Furthermore, to evaluate the physical and biomolecular signaling of the self-assembled microfabricated membranes, rat mesenchymal stem cells are cultured on membranes exhibiting various densities of RGDS and different topographical patterns. Cell adhesion, spreading, and morphology are signifi cantly affected by the surface topographical patterns and the different concentrations of RGDS. The versatility of the combined bottom-up and top-down fabrication processes described may permit the development of hierarchical macrostruc- tures with precise biomolecular and physical properties and the opportunity to fi ne tune them with spatiotemporal control.
dc.format.extent9 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshBiomedical materials
dc.titleCo-assembled and microfabricated bioactive membranes
dc.typeArticle
dc.subject.lemacbiomaterials
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1002/adfm.201201065
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/adfm.201201065/abstract
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac12912495
dc.description.versionPostprint (published version)
dc.date.lift10000-01-01
local.citation.authorMendes, A.; Smith, K.; Tejeda, E.; Engel, E.; Reis, R.; Azevedo, E.; Mata, Á.
local.citation.publicationNameAdvanced functional materials
local.citation.volume28
local.citation.startingPage430
local.citation.endingPage438


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Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain