A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces.
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European Commisision's projectINNOVABONE - Novel biomimetic strategy for bone regeneration (EC-FP7-263363)
THE GRAIL - Tissue in Host Engineering Guided Regeneration of Arterial Intimal Layer (EC-FP7-278557)
We present the immobilization on synthetic substrates of elastin-like recombinamers (ELR) that combine a bioactive motif for cell adhesion with protein antifouling properties. Physical adsorption of the recombinamers and covalent-grafting through organosilane chemistry were investigated. The biochemically-modified surfaces were thoroughly characterized and tested for protein absorption in serum by fluorescence-labelling, XPS, Ellipsometry, and OWLS. The ELR were successfully grafted and stable, even upon mechanical stresses; being the covalent bonding favourable over physical adsorption. The coated metal surfaces exhibited excellent reduction of serum protein adsorption (9 ng/cm2) compared to the bare metal surface (310 ng/cm2). Non-specific protein adsorption may mask the introduced bioactive motifs; therefore, the bioactivated surfaces should display serum-protein antifouling properties. Finally, improved hMSCs response was assessed on the bioactivated substrates. In summary, the coatings simultaneously displayed anti-fouling and bioactive properties. These studies investigated key factors to enhance tissue material interactions fundamental for the design of bioactive devices and future biomedical applications.
CitationSalvagni, E. [et al.]. A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces.. "Colloids and Surfaces B. Biointerfaces", 17 Octubre 2013, vol. 17, núm. 114C, p. 225-233.