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dc.contributor.authorÁlvarez, Zaída
dc.contributor.authorMateos Timoneda, Miguel Ángel
dc.contributor.authorHyroššová, Petra Hyroššová
dc.contributor.authorCastaño Linares, Óscar
dc.contributor.authorPlanell Estany, Josep Anton
dc.contributor.authorPerales, José C.
dc.contributor.authorEngel López, Elisabeth
dc.contributor.authorAlcántara, Soledad
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.contributor.otherInstitut de Bioenginyeria de Catalunya
dc.date.accessioned2013-12-04T09:03:25Z
dc.date.created2013-03-06
dc.date.issued2013-03-06
dc.identifier.citationÁlvarez, Z. [et al.]. The effect of the composition of PLA films and lactate release on glial and neuronal maturation and the maintenance of the neuronal progenitor niche.. "Biomaterials", 06 Març 2013, vol. 34, núm. 9, p. 2221-2233.
dc.identifier.issn0142-9612
dc.identifier.urihttp://hdl.handle.net/2117/20906
dc.description.abstractTo develop tissue engineering strategies useful for repairing damage in the central nervous system (CNS) it is essential to design scaffolds that emulate the NSC niche and its tight control of neural cell genesis, growth, and differentiation. In this study we tested two types of poly l/dl lactic acid (PLA95/5 and PLA70/30), a biodegradable material permissive for neural cell adhesion and growth, as materials for nerve regeneration. Both PLA were slightly hydrophobic and negatively charged but differed in crystallinity, stiffness and degradation rate. PLA95/5 films were highly crystalline, stiff (GPa), and did not degrade significantly in the one-month period analyzed in culture. In contrast, PLA70/30 films were more amorphous, softer (MPa) and degraded faster, releasing significant amounts of lactate into the culture medium. PLA70/30 performs better than PLA95/5 for primary cortical neural cell adhesion, proliferation and differentiation, maintaining the pools of neuronal and glial progenitor cells in vitro. l-lactate in the medium recapitulated PLA70/30's maintenance of neuronal restricted progenitors but did not sustain bipotential or glial restricted progenitors in the cultures, as occurred when neural cells were grown on PLA70/30. Our results suggest that PLA70/30 may mimic some of the physical and biochemical characteristics of the NSC niche. Its mechanical and surface properties may act synergistically in the modulation of bipotential and glial restricted progenitor phenotypes, while it is l-lactate, either added to the medium or released by the film that drives the maintenance of neuronal restricted progenitor cell phenotypes.
dc.format.extent13 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshMaterials--Biodegradation
dc.subject.lcshBiomedical materials
dc.subject.otherPolylactic acid
dc.subject.otherDegradation
dc.subject.otherNeurons
dc.subject.otherProgenitors
dc.subject.otherLactate
dc.subject.otherGlial cells
dc.subject.otherNSC niche
dc.titleThe effect of the composition of PLA films and lactate release on glial and neuronal maturation and the maintenance of the neuronal progenitor niche.
dc.typeArticle
dc.subject.lemacBiodegradació -- Aplicacions en medicina
dc.subject.lemacEnginyeria de teixits
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1016/j.biomaterials.2012.12.001
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0142961212013671#
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac12912562
dc.description.versionPostprint (published version)
dc.date.lift10000-01-01
local.citation.authorÁlvarez, Z.; Mateos-Timoneda, M.A.; Hyroššová, P.; Castaño, O.; Planell, J.; Perales, J.; Engel, E.; Alcántara, S.
local.citation.publicationNameBiomaterials
local.citation.volume34
local.citation.number9
local.citation.startingPage2221
local.citation.endingPage2233


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Attribution-NonCommercial-NoDerivs 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain