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Osteoblast-like cellular response to dynamic changes in the ionic extracellular environment produced by calcium-deficient hydroxyapatite
dc.contributor.author | Gustavsson, Johan |
dc.contributor.author | Ginebra Molins, Maria Pau |
dc.contributor.author | Planell Estany, Josep Anton |
dc.contributor.author | Engel López, Elisabeth |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica |
dc.contributor.other | Institut de Bioenginyeria de Catalunya |
dc.date.accessioned | 2013-06-29T11:11:34Z |
dc.date.created | 2012-10 |
dc.date.issued | 2012-10 |
dc.identifier.citation | Gustavsson, J. [et al.]. Osteoblast-like cellular response to dynamic changes in the ionic extracellular environment produced by calcium-deficient hydroxyapatite. "Journal of materials science. Materials in medicine", Octubre 2012, vol. 23, núm. 10, p. 2509-2520. |
dc.identifier.issn | 0957-4530 |
dc.identifier.uri | http://hdl.handle.net/2117/19727 |
dc.description.abstract | Solution-mediated reactions due to ionic substitutions are increasingly explored as a strategy to improve the biological performance of calcium phosphate-based materials. Yet, cellular response to well-defined dynamic changes of the ionic extracellular environment has so far not been carefully studied in a biomaterials context. In this work, we present kinetic data on how osteoblast-like SAOS-2 cellular activity and calcium-deficient hydroxyapatite (CDHA) influenced extracellular pH as well as extracellular concentrations of calcium and phosphate in standard in vitro conditions. Since cells were grown on membranes permeable to ions and proteins, they could share the same aqueous environment with CDHA, but still be physically separated from the material. In such culture conditions, it was observed that gradual material-induced adsorption of calcium and phosphate from the medium had only minor influence on cellular proliferation and alkaline phosphatase activity, but that competition for calcium and phosphate between cells and the biomaterial delayed and reduced significantly the cellular capacity to deposit calcium in the extracellular matrix. The presented work thus gives insights into how and to what extent solution-mediated reactions can influence cellular response, and this will be necessary to take into account when interpreting CDHA performance both in vitro and in vivo. |
dc.format.extent | 12 p. |
dc.language.iso | eng |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials |
dc.subject | Àrees temàtiques de la UPC::Enginyeria química::Química inorgànica::Compostos inorgànics |
dc.subject.lcsh | Calcium phosphate |
dc.subject.lcsh | Biomedical materials |
dc.subject.other | ALKALINE-PHOSPHATASE ACTIVITY |
dc.subject.other | SAOS-2 CELLS |
dc.subject.other | IN-VITRO |
dc.subject.other | BONE MINERALIZATION |
dc.subject.other | BIOLOGICAL BASIS |
dc.subject.other | DIFFERENTIATION |
dc.subject.other | CULTURE |
dc.subject.other | MATRIX |
dc.subject.other | PROLIFERATION |
dc.subject.other | TOPOGRAPHY |
dc.title | Osteoblast-like cellular response to dynamic changes in the ionic extracellular environment produced by calcium-deficient hydroxyapatite |
dc.type | Article |
dc.subject.lemac | Biomaterials |
dc.subject.lemac | Fosfat de calci |
dc.contributor.group | Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits |
dc.identifier.doi | 10.1007/s10856-012-4705-4 |
dc.rights.access | Restricted access - publisher's policy |
local.identifier.drac | 11109438 |
dc.description.version | Postprint (published version) |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/FP7/214402/EU/Angiogenesis-inducing Bioactive and Bioresponsive Scaffolds in Tissue Enginering/ANGIOSCAFF |
dc.date.lift | 10000-01-01 |
local.citation.author | Gustavsson, J.; Ginebra, M.P.; Planell, J.; Engel, E. |
local.citation.publicationName | Journal of materials science. Materials in medicine |
local.citation.volume | 23 |
local.citation.number | 10 |
local.citation.startingPage | 2509 |
local.citation.endingPage | 2520 |
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