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dc.contributor.authorPsilodimitrakopoulos, Sotiris
dc.contributor.authorAmat Roldán, Iván
dc.contributor.authorLoza Álvarez, Pablo
dc.contributor.authorArtigas García, David
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions
dc.identifier.citationPsilodimitrakopoulos, S. [et al.]. Effect of molecular organization on the image histograms of polarization SHG microscopy. "BIOMEDICAL OPTICS EXPRESS", 2012, vol. 3, núm. 10, p. 2681-2693.
dc.description.abstractBased on its polarization dependency, second harmonic generation (PSHG) microscopy has been proven capable to structurally characterize molecular architectures in different biological samples. By exploiting this polarization dependency of the SHG signal in every pixel of the image, average quantitative structural information can be retrieved in the form of PSHG image histograms. In the present study we experimentally show how the PSHG image histograms can be affected by the organization of the SHG active molecules. Our experimental scenario grounds on two inherent properties of starch granules. Firstly, we take advantage of the radial organization of amylopectin molecules (the SHG source in starch) to attribute shifts of the image histograms to the existence of tilted off the plane molecules. Secondly, we use the property of starch to organize upon hydration to demonstrate that the degree of structural order at the molecular level affects the width of the PSHG image histograms. The shorter the width is the more organized the molecules in the sample are, resulting in a reliable method to measure order. The implication of this finding is crucial to the interpretation of PSHG images used for example in tissue diagnostics.
dc.format.extent13 p.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Enginyeria electrònica::Optoelectrònica
dc.subjectÀrees temàtiques de la UPC::Enginyeria de la telecomunicació::Telecomunicació òptica::Fotònica
dc.subject.otherActive molecules
dc.subject.otherAmylopectin molecules
dc.subject.otherBiological samples
dc.subject.otherDegree of structural order
dc.subject.otherImage histograms
dc.subject.otherMolecular architecture
dc.subject.otherMolecular levels
dc.subject.otherMolecular organization
dc.subject.otherPolarization dependency
dc.subject.otherSHG signals
dc.subject.otherStarch granules
dc.subject.otherStructural information
dc.subject.otherTissue diagnostics
dc.titleEffect of molecular organization on the image histograms of polarization SHG microscopy
dc.contributor.groupUniversitat Politècnica de Catalunya. FOTONICA - Grup de Recerca de Fotònica
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
dc.description.versionPostprint (published version)
local.citation.authorPsilodimitrakopoulos, S.; Amat, I.; Loza, P.; Artigas, D.
local.citation.publicationNameBIOMEDICAL OPTICS EXPRESS

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