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dc.contributor.authorOlate Moya, Felipe
dc.contributor.authorMateos Timoneda, Miguel Ángel
dc.contributor.authorEngel López, Elisabeth
dc.contributor.authorArens, Lukas
dc.contributor.authorWilhelm, Manfred
dc.contributor.authorPalza, Humberto
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials
dc.date.accessioned2020-07-09T13:00:17Z
dc.date.available2021-01-07T01:26:15Z
dc.date.issued2020-01-07
dc.identifier.citationOlate, F. [et al.]. Chondroinductive alginate-based hydrogels having graphene oxide for 3D printed scaffold fabrication. "ACS Applied materials and interfaces", 7 Gener 2020, vol. 12, núm. 4, p. 4343-4357.
dc.identifier.issn1944-8252
dc.identifier.urihttp://hdl.handle.net/2117/192745
dc.description.abstractScaffolds based on bioconjugated hydrogels are attractive for tissue engineering because they can partly mimic human tissue characteristics. For example, they can further increase their bioactivity with cells. However, most of the hydrogels present problems related to their processability, consequently limiting their use in 3D printing to produce tailor-made scaffolds. The goal of this work is to develop bioconjugated hydrogel nanocomposite inks for 3D printed scaffold fabrication through a micro-extrusion process having improved both biocompatibility and processability. The hydrogel is based on a photocrosslinkable alginate bioconjugated with both gelatin and chondroitin sulfate in order to mimic the cartilage extracellular matrix, while the nanofiller is based on graphene oxide to enhance the printability and cell proliferation. Our results show that the incorporation of graphene oxide into the hydrogel inks considerably improved the shape fidelity and resolution of 3D printed scaffolds because of a faster viscosity recovery post extrusion of the ink. Moreover, the nanocomposite inks produce anisotropic threads after the 3D printing process because of the templating of the graphene oxide liquid crystal. The in vitro proliferation assay of human adipose tissue-derived mesenchymal stem cells (hADMSCs) shows that bioconjugated scaffolds present higher cell proliferation than pure alginate, with the nanocomposites presenting the highest values at long times. Live/Dead assay otherwise displays full viability of hADMSCs adhered on the different scaffolds at day 7. Notably, the scaffolds produced with nanocomposite hydrogel inks were able to guide the cell proliferation following the direction of the 3D printed threads. In addition, the bioconjugated alginate hydrogel matrix induced chondrogenic differentiation without exogenous pro-chondrogenesis factors as concluded from immunostaining after 28 days of culture. This high cytocompatibility and chondroinductive effect toward hADMSCs, together with the improved printability and anisotropic structures, makes these nanocomposite hydrogel inks a promising candidate for cartilage tissue engineering based on 3D printing.
dc.format.extent15 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshTissue engineering
dc.subject.lcshGraphene
dc.subject.other3D printing
dc.subject.otherGraphene oxide
dc.subject.otherLiquid crystals
dc.subject.otherHydrogels
dc.subject.otherChondrogenesis
dc.titleChondroinductive alginate-based hydrogels having graphene oxide for 3D printed scaffold fabrication
dc.typeArticle
dc.subject.lemacEnginyeria de teixits
dc.subject.lemacGrafè
dc.contributor.groupUniversitat Politècnica de Catalunya. IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies
dc.identifier.doi10.1021/acsami.9b22062
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acsami.9b22062
dc.rights.accessOpen Access
local.identifier.drac27212855
dc.description.versionPostprint (author's final draft)
local.citation.authorOlate, F.; Mateos, M.; Engel, E.; Arens, L.; Wilhelm, M.; Palza, H.
local.citation.publicationNameACS Applied materials and interfaces
local.citation.volume12
local.citation.number4
local.citation.startingPage4343
local.citation.endingPage4357


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Attribution-NonCommercial-NoDerivs 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain