Biphasic calcium sulfate dihydrate/iron-modified alpha-tricalcium phosphate bone cement for spinal applications : in vitro study
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Document typeArticle
Defense date2010-03
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Abstract
In this study, the cytocompatibility of new ‘iron-modified/alpha-tricalcium phosphate
(IM/α-TCP) and calcium sulfate dihydrate (CSD)’ bone cement (IM/α-TCP/CSD-BC)
intended for spinal applications has been approached. The objective was to investigate by direct-contact osteoblast-like cell cultures (from 1 to 14 days) the in vitro cell adhesion, proliferation, morphology and cytoskeleton organization of MG-63 cells seeded onto the new cements. The results were as follows: (a) quantitative MTT-assay and scanning electron microscopy (SEM) showed that cell adhesion, proliferation and viability were not affected with time by the presence of iron in the cements; (b) double immunofluorescent labeling of F-actin and α-tubulin showed a dynamic interaction between the cell and its porous substrates
sustaining the locomotion phenomenon on the cements’ surface, which favored the
colonization, and confirming the biocompatibility of the experimental cements; (c) SEM-cell morphology and cytoskeleton observations also evidenced that MG-63 cells were able to adhere, to spread and to attain normal morphology on the new IM/α-TCP/CSD-BC which offered favorable substratum properties for osteoblast-like cells proliferation and
differentiation in vitro. The results showed that these new iron-modified cement-like
biomaterials have cytocompatible features of interest not only as possible spinal cancellous bone replacement biomaterial but also as bone tissue engineering scaffolds.
Description
Premi del "National University Research Council" del Ministeri d'Educació i Recerca de Romania, a autors romanesos per l'impacte de la seva recerca.
CitationVlad, M. [et al.]. Biphasic calcium sulfate dihydrate/iron-modified alpha-tricalcium phosphate bone cement for spinal applications : in vitro study. "Biomedical materials", Març 2010, vol. 5, núm. 2, p. 1-13.
Award-winningAward-winning
ISSN1748-6041
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