The integrated use of hydrogeologic and multi-isotopic approaches (d15N, d18ONO3, d34S, d18OSO4 and d13CHCO3) was applied in the Selva basin area (NE Spain) to characterize NO -3 and SO-2 -4 sources and to evaluate which geochemical processes affect NO -3 in groundwater. The studied basin is within a basinand-range physiographic province where natural hydrodynamics have been modified and different scale flow systems converge as a consequence of recent groundwater development and exploitation rates. As a result, groundwaters related to the local recharge flow system (affected by anthropogenic activities) and to the generally deeper regional flow system (recharged from the surrounding ranges) undergo mixing processes. The d15N, d18ONO3 and d34S indicated that the predominant sources of contamination in the basin are pig manure and synthetic fertilizers. Hydrochemical data along with d15N, d18ONO3, d34S, d18OSO4 and d13CHCO3 of some wells confirmed mixing between regional and local flow systems. Apart from dilution processes that can contribute to the decrease of NO -3 concentrations, the positive correlation between d15N and d18ONO3 agreed with the occurrence of denitrification processes. The d34S and d18OSO4 indicated that pyrite oxidation is not linked to denitrification, and d13CHCO3 did not clearly point to a role of organic matter as an electron donor. Therefore, it is proposed that the mixing processes between deeper regional and local surface groundwater allow denitrification to occur due to the reducing conditions of the regional groundwater. Thus, isotopic data add useful complementary information to hydrochemical studies, especially in those areas where hydrochemical data is not conclusive.