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dc.contributor.authorHoyo Pérez, Javier
dc.contributor.authorTorrent Burgués, Juan
dc.contributor.authorTzanov, Tzanko
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.identifier.citationHoyo, J.; Torrent-Burgués, J.; Tzanov, T. Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature. "General Physiology and Biophysics", 26 Març 2020, vol. 39, p. 195-202.
dc.description.abstractThe current strategies to eradicate bacteria require that the antimicrobial agent either penetrate or disrupt the bacterial membrane. In Escherichia coli (E.coli) as a model of Gram-negative strains, the antimicrobials have to cross two barriers – the outer and the inner membrane being the latter composed by ~ 77% phosphatidylethanolamine (PE), ~ 13% phosphatidylglycerol (PG) and ~ 10% cardiolipin (CL) lipids. Each one of these lipid families shares the same headgroup, but contains acyl chains with varying length and degree of unsaturation. Bacteria adapt their membrane lipid composition and metabolism in response to environmental signals, such as the temperature, resulting in different interactions with exogenous molecules, e.g. antibacterial agents. Herein, bacterial model membranes are prepared to evaluate the lipid-lipid interactions in Langmuir monolayers of binary mixtures at several molar ratios of PE and PG or CL at human physiological temperature (37°C). Both PE:PG and PE:CL monolayers were stable at 37°C and presented higher molecular areas (> 20 Å2/molecule) than at 23°C. However, these lipid mixtures presented liquid-expanded state and rigidity (inverse of the compressibility modulus ~ 90 mN/m) slightly lower than at 23°C. Such athermalicity at biologically relevant temperatures may favour the preservation of the biological functions of E.coli
dc.format.extent8 p.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshAnti-infective agents
dc.subject.lcshEscheríchia coli, Infeccions per
dc.subject.lcshCell membranes
dc.subject.otherLipid-lipid interactions
dc.subject.otherBacterial mimetic membranes
dc.subject.otherPhysiological temperature
dc.titleLipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature
dc.subject.lemacAgents antiinfecciosos
dc.subject.lemacInfeccions per escheríchia coli
dc.subject.lemacMembranes cel·lulars
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/H2020/720851/EU/Pre-commercial lines for production of surface nanostructured antimicrobial and anti-biofilm textiles, medical devices and water treatment membranes/PROTECT
local.citation.authorHoyo, J.; Torrent-Burgués, J.; Tzanov, T.
local.citation.publicationNameGeneral Physiology and Biophysics

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