Development of an alginate-based hydrogel loaded with multifuncional gold nanoparticles for regenerative therapies
Document typeMaster thesis
Rights accessOpen Access
The aim of this project was to develop a multifunctional alginate-based hydrogel modified with peptide-functionalized gold nanoparticles (AuNPs). Hydrogels are versatile materials, commonly used in tissue engineering due to their biocompatibility and tunable properties. In detail, this project explored the modification of alginate hydrogels with functionalized AuNPs incorporating bioactive peptides, such as LF1-11 or RGD, which are known for preventing bacterial infection and promoting cell adhesion, respectively. To this end, this project focused on the synthesis of AuNPs, their conjugation with peptides and, finally, the hybrid hydrogel preparation. In order to optimize and characterize the fabrication process, several physicochemical techniques were used such as UV-visible spectroscopy, transmission electron microscopy or X-ray photoelectron spectroscopy. The synthesis of the hybrid biomaterial was successfully executed and optimized at each step. The fabrication of this hybrid hydrogel proved to be a robust procedure and highly reproducible. Interestingly, the presence of the antimicrobial peptide LF1-11 decreased the adhesion of bacteria to the hydrogel. Further bacterial and cellular assays are thus warranted in order to ascertain the potential applications of this new biomaterial, e.g. as a film/dressing for infected and chronic wounds or injectable gel for bone defects.