Preliminary studies for cell-laden hydrogels developement
Tutor / director / evaluatorCiardelli, Gianluca
CovenanteePolitecnico di Torino
Document typeMaster thesis
Rights accessOpen Access
The following document presents a Master’s Thesis based on preliminary studies for the development of cell-laden hydrogels and their biological characterisation. The aim of this hydrogels elaboration is the peripheral nerve regeneration, in this case mainly related to the regeneration of the sense of touch. The results obtained through the experimentation of cells encapsulation would serve to know which are the best conditions and the preferable environments where cells can live and reproduce. It would definitely set a step forward for the in vivo studies and the real regeneration of the peripheral nerves. In first place, there is a preface, where the main reasons to develop this project and the importance that hydrogels have in the current investigation for tissue regeneration are explained. In second place, the objectives of this project and its scope are defined. Then, an introduction and description of the materials being used in the experiments is done. The developed hydrogels are chitosan based with a phosphate salt as gelling agent. The cells being used for the encapsulation are Schwann cells, which are the cells in the peripheral nervous system, residing at sensory nerve endings. Next, the summarisation of the executed experiments can be found. The first point made is the description of the obtaining process of hydrogels and the characterisation of the selected ones for doing the cell encapsulation, while the second point made is the description of the experimentation with cells, its complications and results. The hydrogels finally obtained will be used in further investigations and this project will give an idea from where to focus the attention in the future and on how to continue with the experimentation. The hydrogels characterisation and the experiments done at the Politecnico di Torino brought to light the best hydrogel in terms of gelation time, stability and pH are the CS 95/100 2.5% w/v R=4 with β-GP and the CS 95/100 2% w/v R=4.5 with G1-P. The experimentation with cells developed in San Luigi Hospital, in Orbassano, Torino, were based in the study of the behaviour of cells to these two hydrogels and its components. The results showed that the hydrogel CS 95/100 2.5% w/v R=4 with β-GP presents a better response in the experiments of cells encapsulation and cells on the top, for the concentrations defined. On the contrary, the hydrogel CS 95/100 2% w/v R=4.5 with G1-P presents better cells behaviour when working with lower concentrations of salt. Further investigations will be performed to define the optimal hydrogel composition.
SubjectsBiomedical materials, Tissue engineering, Biomedical engineering, Materials biomèdics, Nervis perifèrics, Enginyeria de teixits, Enginyeria biomèdica
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