Depth aberrations characterization in linear and nonlinear microscopy schemes using a Shack-Hartmann wavefront sensor
Visualitza/Obre
8227_49.pdf (1,674Mb) (Accés restringit)
Sol·licita una còpia a l'autor
Què és aquest botó?
Aquest botó permet demanar una còpia d'un document restringit a l'autor. Es mostra quan:
- Disposem del correu electrònic de l'autor
- El document té una mida inferior a 20 Mb
- Es tracta d'un document d'accés restringit per decisió de l'autor o d'un document d'accés restringit per política de l'editorial
Cita com:
hdl:2117/18194
Tipus de documentText en actes de congrés
Data publicació2012
Condicions d'accésAccés restringit per política de l'editorial
Tots els drets reservats. Aquesta obra està protegida pels drets de propietat intel·lectual i
industrial corresponents. Sense perjudici de les exempcions legals existents, queda prohibida la seva
reproducció, distribució, comunicació pública o transformació sense l'autorització del titular dels drets
Abstract
The performance of imaging devices such as linear and nonlinear microscopes (NLM) can be limited by the optical properties of the imaged sample. Such an important aspect has already been described using theoretical models due to the difficulties of implementing a direct wavefront sensing scheme. However, these only stand for simple interfaces and cannot be generalized to biological samples given its structural complexity. This has leaded to the development of sensor-less adaptive optics (AO) implementations. In this approach, aberrations are iteratively corrected trough an image related parameter (aberrations are not measured), being prone of causing sample damage. In this work, we perform a practical implementation of a Shack-Hartman wavefront sensor to compensate for sample induced aberrations, demonstrating its applicability in linear and NLM. We perform an extensive analysis of wavefront distortion effects through different depths employing phantom samples. Aberration effects originated by the refractive index mismatch and depth are quantified using the linear and nonlinear guide-star concept. More over we analyze offaxis aberrations in NLM, an important aspect that is commonly overlooked. In this case spherical aberration behaves similarly to the wavefront error compared with the on-axis case. Finally we give examples of aberration compensation using epi-fluorescence and nonlinear microscopy.
CitacióAviles, R. [et al.]. Depth aberrations characterization in linear and nonlinear microscopy schemes using a Shack-Hartmann wavefront sensor. A: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing. "Progress in Biomedical Optics and Imaging - Proceedings of SPIE". 2012, p. 82271D-1-82271D-11.
ISBN978-081948870-1
Fitxers | Descripció | Mida | Format | Visualitza |
---|---|---|---|---|
8227_49.pdf | 1,674Mb | Accés restringit |