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Bacterial cellulose matrices to develop enzymatically active paper
dc.contributor.author | Buruaga Ramiro, Carolina |
dc.contributor.author | Valenzuela, Susana V. |
dc.contributor.author | Valls Vidal, Cristina |
dc.contributor.author | Roncero Vivero, María Blanca |
dc.contributor.author | Javier Pastor, F.I. |
dc.contributor.author | Diaz Lucea, M. Pilar |
dc.contributor.author | Martínez Martínez, Josefina |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament d'Expressió Gràfica a l'Enginyeria |
dc.date.accessioned | 2020-03-19T11:48:28Z |
dc.date.available | 2021-01-31T01:25:49Z |
dc.date.issued | 2020-01-31 |
dc.identifier.citation | Buruaga, C. [et al.]. Bacterial cellulose matrices to develop enzymatically active paper. "Cellulose", 31 Gener 2020, vol. 27, p. 3413-3426. |
dc.identifier.issn | 0969-0239 |
dc.identifier.uri | http://hdl.handle.net/2117/180519 |
dc.description.abstract | This work studies the suitability of bacterial cellulose (BC) matrices to prepare enzymatically active nanocomposites, in a framework of more environmentally friendly methodologies. After BC production and purification, two kind of matrices were obtained: BC in aqueous suspension and BC paper. A lipase was immobilised onto the BC matrices by physical adsorption, obtaining Lipase/BC nanocomposites. Neither morphology nor crystallinity, measured by scanning electron microscopy and X-ray diffractometry respectively, of the BC were affected by the binding of the protein. The activity of Lipase/BC suspension and Lipase/BC paper was tested under different conditions, and the operational properties of the enzyme were evaluated. A shift towards higher temperatures, a broader pH activity range, and slight differences in the substrate preference were observed in the immobilised lipase, compared with the free enzyme. Specific activity was higher for Lipase/BC suspension (4.2 U/mg) than for Lipase/BC paper (1.7 U/mg) nanocomposites. However, Lipase/BC paper nanocomposites showed improved thermal stability, reusability, and durability. Enzyme immobilised onto BC paper retained 60% of its activity after 48 h at 60 °C. It maintained 100% of the original activity after being recycled 10 times at pH 7 at 60 °C and it remained active after being stored for more than a month at room temperature. The results suggested that lipase/BC nanocomposites are promising biomaterials for the development of green biotechnological devices with potential application in industrials bioprocesses of detergents and food industry and biomedicine. Lipase/BC paper nanocomposite might be a key component of bioactive paper for developing simple, handheld, and disposable devices |
dc.format.extent | 14 p. |
dc.language.iso | eng |
dc.subject | Àrees temàtiques de la UPC::Enginyeria paperera |
dc.subject.lcsh | Cellulose |
dc.subject.lcsh | Nanocomposites (Materials) |
dc.subject.lcsh | Lipase |
dc.subject.lcsh | Adsorption |
dc.subject.other | Bacterial cellulose |
dc.subject.other | Lipase immobilization |
dc.subject.other | Physical adsorption |
dc.subject.other | Nanocomposite |
dc.subject.other | Bacterial cellulose biopaper |
dc.title | Bacterial cellulose matrices to develop enzymatically active paper |
dc.type | Article |
dc.subject.lemac | Cel·lulosa |
dc.subject.lemac | Lipases |
dc.subject.lemac | Nanocompòsits (Materials) |
dc.subject.lemac | Adsorció |
dc.contributor.group | Universitat Politècnica de Catalunya. CELBIOTECH - Grup de Recerca: Enginyeria Paperera |
dc.identifier.doi | 10.1007/s10570-020-03025-9 |
dc.relation.publisherversion | https://link.springer.com/article/10.1007/s10570-020-03025-9 |
dc.rights.access | Open Access |
local.identifier.drac | 27211770 |
dc.description.version | Postprint (author's final draft) |
local.citation.author | Buruaga, C.; Valenzuela, S.V.; Valls, C.; Roncero, M.B.; Javier, F.; Diaz Lucea, M. Pilar; Martínez Martínez, Josefina |
local.citation.publicationName | Cellulose |
local.citation.volume | 27 |
local.citation.startingPage | 3413 |
local.citation.endingPage | 3426 |
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