Room Temperature Excitation-Emission Spectra of Single LH2 Complexes Show Remarkably Little Variation
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acs.jpclett.0c00375.pdf (733,0Kb) (Accés restringit)
10.1021/acs.jpclett.0c00375
Inclou dades d'ús des de 2022
Cita com:
hdl:2117/179411
Tipus de documentArticle
Data publicació2020-03-06
EditorACS
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Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
ProjecteACOPLAMIENTO OPTICO RESONANTE GIGANTE (AEI-PGC2018-096875-B-I00)
AGR-INSTITUTO DE CIENCIAS FOTONICAS (MINECO-SEV-2015-0522)
FEMTONANO : VISTAS ULTRARRAPIDAS DE TRANSPORTE CUANTICO EN NANO-REDES FOTONICAS NATURALES (MINECO-FIS2015-69258-P)
AGR-INSTITUTO DE CIENCIAS FOTONICAS (MINECO-SEV-2015-0522)
FEMTONANO : VISTAS ULTRARRAPIDAS DE TRANSPORTE CUANTICO EN NANO-REDES FOTONICAS NATURALES (MINECO-FIS2015-69258-P)
Abstract
Excitation spectroscopy gives direct insight in excited state manifold, energy transfer,
transient intermediates, vibrations, etc. Unfortunately, excitation spectroscopy of single
molecules at ambient conditions has remained challenging. Here, we present excitation
spectra alongside emission spectra of the same individual light harvesting complex LH2
of the purple bacteria Rps. acidophila. Acquisition of both the excited and ground state
spectra allows to quantify disorder and interband correlations, which are key variables for
the interpretation of observed long-lasting coherences. We have overcome the low
photostability and small fluorescence quantum yield, inherent to many biologically
relevant systems, by combining single molecule Fourier transform spectroscopy, low
excitation intensities, and effective data analysis. We find that LH2 complexes show little
spectral variation (130-170 cm-1), that their two absorption bands (B800-B850) act
uncorrelated, and that the Stokes shift is not constant. The low amount of spectral
disorder underlines the protective role of the protein scaffold, benefitting the efficient
energy transport throughout the light-harvesting membrane
CitacióGellings, E.; Cogdell, R. J.; Hulst, N. F. V. Room Temperature Excitation-Emission Spectra of Single LH2 Complexes Show Remarkably Little Variation. "", 6 Març 2020.
Versió de l'editorhttps://pubs.acs.org/doi/10.1021/acs.jpclett.0c00375
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