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dc.contributor.authorLopez Laguna, Hector
dc.contributor.authorCubarsí Morera, Rafael
dc.contributor.authorUnzueta, Ugutz
dc.contributor.authorMangues, Ramón
dc.contributor.authorVazquez, Esther
dc.contributor.authorVillaverde, Antonio
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Matemàtiques
dc.date.accessioned2020-03-04T13:05:17Z
dc.date.available2020-03-04T13:05:17Z
dc.date.issued2019-12-27
dc.identifier.citationLopez-Laguna, H. [et al.]. Endosomal escape of protein nanoparticles engineered through humanized histidine-rich peptides. "SCIENCE CHINA Materials", April 2020, vol. 63, núm. 4, p. 644-653.
dc.identifier.otherhttps://link.springer.com/content/pdf/10.1007/s40843-019-1231-y.pdf
dc.identifier.urihttp://hdl.handle.net/2117/179196
dc.description.abstractPoly-histidine peptides such as H6 (HHHHHH) are used in protein biotechnologies as purification tags, pro- tein-assembling agents and endosomal-escape entities. The pleiotropic properties of such peptides make them appealing to design protein-based smart materials or nanoparticles for imaging or drug delivery to be produced in form of re- combinant proteins. However, the clinical applicability of H6- tagged proteins is restricted by the potential immunogenicity of these segments. In this study, we have explored several humanized histidine-rich peptides in tumor-targeted modular proteins, which can specifically bind and be internalized by the target cells through the tumoral marker CXCR4. We were particularly interested in exploring how protein purification, self-assembling and endosomal escape perform in proteins containing the variant histidine-rich tags. Among the tested candidates, the peptide H5E (HEHEHEHEH) is promising as a good promoter of endosomal escape of the associated full- length protein upon endosomal internalization. The numer- ical modelling of cell penetration and endosomal escape of the tested proteins has revealed a negative relationship between the amount of protein internalized into target cells and the efficiency of cytoplasmic release. This fact demonstrates that the His-mediated, proton sponge-based endosomal escape saturates at moderate amounts of internalized protein, a fact that might be critical for the design of protein materials for cytosolic molecular delivery.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Matemàtiques i estadística::Matemàtica aplicada a les ciències
dc.subject.lcshBiomathematics
dc.subject.otherprotein materials
dc.subject.othernanoparticles
dc.subject.othergenetic design
dc.subject.otherendosomal escape
dc.subject.otherpoly-histidines
dc.titleEndosomal escape of protein nanoparticles engineered through humanized histidine-rich peptides
dc.typeArticle
dc.subject.lemacBiomatemàtica
dc.identifier.doi10.1007/s40843-019-1231-y
dc.description.peerreviewedPeer Reviewed
dc.subject.amsClassificació AMS::92 Biology and other natural sciences::92B Mathematical biology in general
dc.relation.publisherversionhttp://engine.scichina.com/doi/10.1007/s40843-019-1231-y
dc.rights.accessOpen Access
local.identifier.drac26406840
dc.description.versionPostprint (author's final draft)
local.citation.authorLopez-Laguna, H.; Cubarsi, R.; Unzueta, U.; Mangues, R.; Vazquez, E.; Villaverde, A.
local.citation.publicationNameSCIENCE CHINA Materials
local.citation.volume63
local.citation.number4
local.citation.startingPage644
local.citation.endingPage653


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