Influence of electrical stimulation on 3D-cultures of Adipose Tissue Derived Progenitor Cells (ATDPCs) behavior
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Tissue engineering has a fundamental role in regenerative medicine. Still today, the major motivation for cardiac regeneration is to design a platform that enables the complete tissue structure and physiological function regeneration of injured myocardium areas. Although tissue engineering approaches have been generally developed for two-dimensional (2D) culture systems, three-dimensional (3D) systems are being spotlighted as the means to mimic better in vivo cellular conditions. This manuscript examines the influence of electrical stimulation on 3D cultures of adipose tissue-derived progenitor cells (ATDPCs). ATDPCs cells were encapsulated into a self-assembling peptide nanoscaffold (RAD16-I) and continuously electro stimulated during 14-20 days with 2-ms pulses of 50mV/cm at a frequency of 1 Hz. Good cellular network formation and construct diameter reduction was observed in electro stimulated samples. Importantly, the process of electro stimulation does not disrupt cell viability or connectivity. As a future outlook, differentiation studies to cardiomyocytes-like cells will be performed analyzing gene profile and protein expression.
CitationCastells-Sala, C. [et al.]. Influence of electrical stimulation on 3D-cultures of Adipose Tissue Derived Progenitor Cells (ATDPCs) behavior. A: IEEE Engineering in Medicine and Biology Society. "34th Annual International Conference of the IEEE EMBS San Diego, California USA, 28 August-1 September, 2012". San Diego, California: IEEE, 2012, p. 5658-5661.
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