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dc.contributor.authorSadowska, Joanna Maria
dc.contributor.authorWei, Fei
dc.contributor.authorGuo, Jia
dc.contributor.authorGuillem Martí, Jordi
dc.contributor.authorLin, Zhengmei
dc.contributor.authorGinebra Molins, Maria Pau
dc.contributor.authorXiao, Yin
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials
dc.identifier.citationSadowska, J. [et al.]. The effect of biomimetic calcium deficient hydroxyapatite and sintered ß-tricalcium phosphate on osteoimmune reaction and osteogenesis. "Acta biomaterialia", 15 Setembre 2019, vol. 96, p. 605-618.
dc.description.abstractBiomaterial implantation triggers inflammatory reactions. Understanding the effect of physicochemical features of biomaterials on the release of inflammatory cytokines from immune cells would be of great interest in view of designing bone graft materials to enhance the healing of bone defects. The present work investigated the interactions of two chemically and texturally different calcium phosphate (CaPs) substrates with macrophages, one of the main innate immune cells, and its further impact on osteogenic differentiation of bone forming cells. The behaviour of macrophages seeded on biomimetic calcium deficient hydroxyapatite (CDHA) and sintered ß-tricalcium phosphate (ß-TCP) was assessed in terms of the release of inflammatory cytokines and osteoclastogenic factors. The osteogenic differentiation of bone progenitor cells (bone marrow stromal cells (BMSCs) and osteoblastic cell line (SaOS-2)) were subsequently studied by incubating with the conditioned medium induced by macrophage-CaPs interaction in order to reveal the effect of immune cell reaction to CaPs on osteogenic differentiation. It was found that the incubation of macrophages with CaPs substrates caused a decrease of pro-inflammatory cytokines, more pronounced for ß-TCP compared with CDHA showing significantly decreased IL-6, TNF-a, and iNOS. However, the macrophage-CDHA interaction resulted in a more favourable environment for osteogenic differentiation of osteoblasts with more collagen type I production and osteogenic genes (Runx2, BSP) expression, suggesting that osteogenic differentiation of bone cells is not only determined by the nature of biomaterials, but also significantly influenced by the inflammatory environment generated by the interaction of immune cells and biomaterials.
dc.format.extent14 p.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
dc.subject.lcshBiomedical materials
dc.subject.otherCalcium phosphates
dc.titleThe effect of biomimetic calcium deficient hydroxyapatite and sintered ß-tricalcium phosphate on osteoimmune reaction and osteogenesis
dc.subject.lemacMaterials biomèdics
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
dc.description.versionPostprint (author's final draft)
dc.contributor.covenanteeInstitute of Health and Biomedical Innovation
dc.contributor.covenanteeAustralia-China Centre for Tissue Engineering and Regenerative Medicine
dc.contributor.covenanteeInstitut de Bioenginyeria de Catalunya
local.citation.authorSadowska, J.; Wei, F.; Guo, J.; Guillem-Marti, J.; Lin, Z.; Ginebra, M.P.; Xiao, Y.
local.citation.publicationNameActa biomaterialia

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