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dc.contributor.authorDíez Escudero, Anna
dc.contributor.authorTorreggiani, E.
dc.contributor.authorPompo, Gemma di
dc.contributor.authorEspañol Pons, Montserrat
dc.contributor.authorPersson, Cecilia
dc.contributor.authorCiapetti, Gabriela
dc.contributor.authorBaldini, Nicola
dc.contributor.authorGinebra Molins, Maria Pau
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials
dc.date.accessioned2020-02-05T10:02:32Z
dc.date.available2020-02-05T10:02:32Z
dc.date.issued2019-01-01
dc.identifier.citationDiez-Escudero, A. [et al.]. Effect of calcium phosphate heparinization on the in vitro inflammatory response and osteoclastogenesis of human blood precursor cells. "Journal of tissue engineering and regenerative medicine", 1 Gener 2019, vol. 13, núm. 7, p. 1217-1229.
dc.identifier.issn1932-6254
dc.identifier.urihttp://hdl.handle.net/2117/176812
dc.description.abstractThe immobilization of natural molecules on synthetic bone grafts stands as a strategy to enhance their biological interactions. During the early stages of healing, immune cells and osteoclasts (OC) modulate the inflammatory response and resorb the biomaterial, respectively. In this study, heparin, a naturally occurring molecule in the bone extracellular matrix, was covalently immobilized on biomimetic calcium-deficient hydroxyapatite (CDHA). The effect of heparin-functionalized CDHA on inflammation and osteoclastogenesis was investigated using primary human cells and compared with pristine CDHA and beta-tricalcium phosphate (ß-TCP). Biomimetic substrates led to lower oxidative stresses by neutrophils and monocytes than sintered ß-TCP, even though no further reduction was induced by the presence of heparin. In contrast, heparinized CDHA fostered osteoclastogenesis. Optical images of stained TRAP positive cells showed an earlier and higher presence of multinucleated cells, compatible with OC at 14 days, while pristine CDHA and ß-TCP present OC at 21–28 days. Although no statistically significant differences were found in the OC activity, microscopy images evidenced early stages of degradation on heparinized CDHA, compatible with osteoclastic resorption. Overall, the results suggest that the functionalization with heparin fostered the formation and activity of OC, thus offering a promising strategy to integrate biomaterials in the bone remodelling cycle by increasing their OC-mediated resorption.
dc.format.extent13 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
dc.subject.lcshBiomedical materials
dc.subject.otherBiomaterial
dc.subject.otherHeparin
dc.subject.otherHydroxyapatite
dc.subject.otherInflammation
dc.subject.otherOsteoclastogenesis
dc.titleEffect of calcium phosphate heparinization on the in vitro inflammatory response and osteoclastogenesis of human blood precursor cells
dc.typeArticle
dc.subject.lemacMaterials biomèdics
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1002/term.2872
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/term.2872
dc.rights.accessOpen Access
local.identifier.drac25176540
dc.description.versionPostprint (author's final draft)
dc.contributor.covenanteeIstituto Ortopedico Rizzoli
dc.contributor.covenanteeUppsala universitet
dc.contributor.covenanteeUniversità di Bologna
dc.contributor.covenanteeInstitut de Bioenginyeria de Catalunya
local.citation.authorDiez-Escudero, A.; Torreggiani, E.; Pompo, G.; Español, M.; Persson, C.; Ciapetti, G.; Baldini, N.; Ginebra, M.P.
local.citation.publicationNameJournal of tissue engineering and regenerative medicine
local.citation.volume13
local.citation.number7
local.citation.startingPage1217
local.citation.endingPage1229


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