Ir al contenido (pulsa Retorno)

Universitat Politècnica de Catalunya

    • Català
    • Castellano
    • English
    • LoginRegisterLog in (no UPC users)
  • mailContact Us
  • world English 
    • Català
    • Castellano
    • English
  • userLogin   
      LoginRegisterLog in (no UPC users)

UPCommons. Global access to UPC knowledge

Banner header
69.147 UPC E-Prints
You are here:
View Item 
  •   DSpace Home
  • E-prints
  • Departaments
  • Departament de Ciència i Enginyeria de Materials
  • Articles de revista
  • View Item
  •   DSpace Home
  • E-prints
  • Departaments
  • Departament de Ciència i Enginyeria de Materials
  • Articles de revista
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Effect of calcium phosphate heparinization on the in vitro inflammatory response and osteoclastogenesis of human blood precursor cells

Thumbnail
View/Open
Postprint -DRAC.pdf (1,283Mb)
 
10.1002/term.2872
 
  View UPCommons Usage Statistics
  LA Referencia / Recolecta stats
Includes usage data since 2022
Cita com:
hdl:2117/176812

Show full item record
Díez Escudero, AnnaMés informacióMés informacióMés informació
Torreggiani, E.
Pompo, Gemma di
Español Pons, MontserratMés informacióMés informacióMés informació
Persson, Cecilia
Ciapetti, Gabriela
Baldini, Nicola
Ginebra Molins, Maria PauMés informacióMés informacióMés informació
CovenanteeIstituto Ortopedico Rizzoli; Uppsala universitet; Università di Bologna; Institut de Bioenginyeria de Catalunya
Document typeArticle
Defense date2019-01-01
Rights accessOpen Access
Attribution-NonCommercial-NoDerivs 3.0 Spain
This work is protected by the corresponding intellectual and industrial property rights. Except where otherwise noted, its contents are licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain
Abstract
The immobilization of natural molecules on synthetic bone grafts stands as a strategy to enhance their biological interactions. During the early stages of healing, immune cells and osteoclasts (OC) modulate the inflammatory response and resorb the biomaterial, respectively. In this study, heparin, a naturally occurring molecule in the bone extracellular matrix, was covalently immobilized on biomimetic calcium-deficient hydroxyapatite (CDHA). The effect of heparin-functionalized CDHA on inflammation and osteoclastogenesis was investigated using primary human cells and compared with pristine CDHA and beta-tricalcium phosphate (ß-TCP). Biomimetic substrates led to lower oxidative stresses by neutrophils and monocytes than sintered ß-TCP, even though no further reduction was induced by the presence of heparin. In contrast, heparinized CDHA fostered osteoclastogenesis. Optical images of stained TRAP positive cells showed an earlier and higher presence of multinucleated cells, compatible with OC at 14 days, while pristine CDHA and ß-TCP present OC at 21–28 days. Although no statistically significant differences were found in the OC activity, microscopy images evidenced early stages of degradation on heparinized CDHA, compatible with osteoclastic resorption. Overall, the results suggest that the functionalization with heparin fostered the formation and activity of OC, thus offering a promising strategy to integrate biomaterials in the bone remodelling cycle by increasing their OC-mediated resorption.
CitationDiez-Escudero, A. [et al.]. Effect of calcium phosphate heparinization on the in vitro inflammatory response and osteoclastogenesis of human blood precursor cells. "Journal of tissue engineering and regenerative medicine", 1 Gener 2019, vol. 13, núm. 7, p. 1217-1229. 
URIhttp://hdl.handle.net/2117/176812
DOI10.1002/term.2872
ISSN1932-6254
Publisher versionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/term.2872
Collections
  • Departament de Ciència i Enginyeria de Materials - Articles de revista [914]
  • BBT - Grup de recerca en Biomaterials, Biomecànica i Enginyeria de Teixits - Articles de revista [480]
  View UPCommons Usage Statistics

Show full item record

FilesDescriptionSizeFormatView
Postprint -DRAC.pdf1,283MbPDFView/Open

Browse

This CollectionBy Issue DateAuthorsOther contributionsTitlesSubjectsThis repositoryCommunities & CollectionsBy Issue DateAuthorsOther contributionsTitlesSubjects

© UPC Obrir en finestra nova . Servei de Biblioteques, Publicacions i Arxius

info.biblioteques@upc.edu

  • About This Repository
  • Metadata under:Metadata under CC0
  • Contact Us
  • Send Feedback
  • Privacy Settings
  • Inici de la pàgina