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A dual molecular biointerface combining RGD and KRSR sequences improves osteoblastic functions by synergizing integrin and cell-membrane proteoglycan binding

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ijms-20-01429 (3).pdf (2,112Mb)
 
10.3390/ijms20061429
 
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Hoyos Nogués, MireiaMés informació
Falgueras Batlle, Elena
Ginebra Molins, Maria PauMés informacióMés informacióMés informació
Manero Planella, José MaríaMés informacióMés informacióMés informació
Gil Mur, Francisco JavierMés informacióMés informacióMés informació
Mas Moruno, CarlosMés informacióMés informacióMés informació
CovenanteeUniversitat Internacional de Catalunya
Document typeArticle
Defense date2019-03-21
Rights accessOpen Access
Attribution-NonCommercial-NoDerivs 3.0 Spain
This work is protected by the corresponding intellectual and industrial property rights. Except where otherwise noted, its contents are licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain
ProjectRECUBRIMIENTOS OSTEOINDUCTIVOS Y ANTIMICROBIANOS AVANZADOS PARA MEJORAR LA OSTEOINTEGRACION DE BIOMATERIALES EN PATOLOGIAS OSTEOPOROTICAS Y DIABETICAS (MINECO-MAT2015-67183-R)
SUPERFICIES MULTIFUNCIONALES BIOMIMETICAS: UNA NUEVA ESTRATEGIA BIOMOLECULAR PARA CONTROLAR LA RESPUESTA CELULAR EN TERAPIAS REGENERATIVAS (AEI-MAT2017-83905-R)
Abstract
Synergizing integrin and cell-membrane heparan sulfate proteoglycan signaling on biomaterials through peptidic sequences is known to have beneficial effects in the attachment and behavior of osteoblasts; however, controlling the exact amount and ratio of peptides tethered on a surface is challenging. Here, we present a dual molecular-based biointerface combining integrin (RGD) and heparin (KRSR)-binding peptides in a chemically controlled fashion. To this end, a tailor-made synthetic platform (PLATF) was designed and synthesized by solid-phase methodologies. The PLATF and the control linear peptides (RGD or KRSR) were covalently bound to titanium via silanization. Physicochemical characterization by means of contact angle, Raman spectroscopy and XPS proved the successful and stable grafting of the molecules. The biological potential of the biointerfaces was measured with osteoblastic (Saos-2) cells both at short and long incubation periods. Biomolecule grafting (either the PLATF, RGD or KRSR) statistically improved (p < 0.05) cell attachment, spreading, proliferation and mineralization, compared to control titanium. Moreover, the molecular PLATF biointerface synergistically enhanced mineralization (p < 0.05) of Saos-2 cells compared to RGD or KRSR alone. These results indicate that dual-function coatings may serve to improve the bioactivity of medical implants by mimicking synergistic receptor binding.
CitationHoyos, M. [et al.]. A dual molecular biointerface combining RGD and KRSR sequences improves osteoblastic functions by synergizing integrin and cell-membrane proteoglycan binding. "International journal of molecular sciences", 21 Març 2019, vol. 20, núm. 6, p. 1429:1-1429:13. 
URIhttp://hdl.handle.net/2117/176143
DOI10.3390/ijms20061429
ISSN1422-0067
Publisher versionhttps://www.mdpi.com/1422-0067/20/6/1429
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  • Departament de Ciència i Enginyeria de Materials - Articles de revista [914]
  • CRnE - Centre de Recerca en Ciència i Enginyeria Multiescala de Barcelona - Articles de revista [6]
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