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Poly-L/DL-lactic acid films functionalized with collagen IV as carrier substrata for corneal epithelial stem cells
dc.contributor.author | de la Mata Sampedro, Ana |
dc.contributor.author | Mateos Timoneda, Miguel Ángel |
dc.contributor.author | Nieto Miguel, Teresa |
dc.contributor.author | Galindo de la Rosa, Sara |
dc.contributor.author | López Paniagua, Marina |
dc.contributor.author | Planell Estany, Josep Anton |
dc.contributor.author | Engel López, Elisabeth |
dc.contributor.author | Calonge Cano, Margarita |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials |
dc.date.accessioned | 2020-01-28T13:50:42Z |
dc.date.available | 2021-05-31T00:31:33Z |
dc.date.issued | 2019-05-01 |
dc.identifier.citation | de la Mata, A. [et al.]. Poly-L/DL-lactic acid films functionalized with collagen IV as carrier substrata for corneal epithelial stem cells. "Colloids and Surfaces B: Biointerfaces", 1 Maig 2019, vol. 177, núm. May, p. 121-129. |
dc.identifier.issn | 1873-4367 |
dc.identifier.uri | http://hdl.handle.net/2117/175946 |
dc.description.abstract | Limbal epithelial stem cells (LESCs) are responsible for the renewal of corneal epithelium. Cultivated limbal epithelial transplantation is the current treatment of choice for restoring the loss or dysfunction of LESCs. To perform this procedure, a substratum is necessary for in vitro culturing of limbal epithelial cells and their subsequent transplantation onto the ocular surface. In this work, we evaluated poly-L/DL-lactic acid 70:30 (PLA) films functionalized with type IV collagen (col IV) as potential in vitro carrier substrata for LESCs. We first demonstrated that PLA-col IV films were biocompatible and suitable for the proliferation of human corneal epithelial cells. Subsequently, limbal epithelial cell suspensions, isolated from human limbal rings, were cultivated using culture medium that did not contain animal components. The cells adhered significantly faster to PLA-col IV films than to tissue culture plastic (TCP). The mRNA expression levels for the LESC specific markers, K15, P63a and ABCG2 were similar or greater (significantly in the case of K15) in limbal epithelial cells cultured on PLA-col IV films than limbal epithelial cells cultured on TCP. The percentage of cells expressing the corneal (K3, K12) and the LESC (P63a, ABCG2) specific markers was similar for both substrata. These results suggest that the PLA-col IV films promoted LESC attachment and helped to maintain their undifferentiated stem cell phenotype. Consequently, these substrata offer an alternative for the transplantation of limbal cells onto the ocular surface. |
dc.format.extent | 9 p. |
dc.language.iso | eng |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials |
dc.subject.lcsh | Biophysics |
dc.subject.other | Corneal epithelium |
dc.subject.other | Collagen IV |
dc.subject.other | Limbal stem cells |
dc.subject.other | Polylactic acid |
dc.subject.other | Tissue engineering |
dc.title | Poly-L/DL-lactic acid films functionalized with collagen IV as carrier substrata for corneal epithelial stem cells |
dc.type | Article |
dc.subject.lemac | Biofísica |
dc.contributor.group | Universitat Politècnica de Catalunya. IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies |
dc.identifier.doi | 10.1016/j.colsurfb.2019.01.054 |
dc.description.peerreviewed | Peer Reviewed |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0927776519300542 |
dc.rights.access | Open Access |
local.identifier.drac | 23943432 |
dc.description.version | Postprint (author's final draft) |
local.citation.author | de la Mata, A.; Mateos, M.; Nieto, T.; Galindo, S.; Lopez, M.; Planell, J. A.; Engel, E.; Calonge, M. |
local.citation.publicationName | Colloids and Surfaces B: Biointerfaces |
local.citation.volume | 177 |
local.citation.number | May |
local.citation.startingPage | 121 |
local.citation.endingPage | 129 |
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