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dc.contributor.authorGuimerà Villalba, Xavier
dc.contributor.authorMoya Lara, Ana
dc.contributor.authorDorado Castaño, Antonio David
dc.contributor.authorIlla, Xavi
dc.contributor.authorVilla, Rosa
dc.contributor.authorGabriel Buguña, David
dc.contributor.authorGamisans Noguera, Javier
dc.contributor.authorGabriel Buguña, Gemma
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Minera, Industrial i TIC
dc.date.accessioned2020-01-15T09:53:48Z
dc.date.available2020-01-15T09:53:48Z
dc.date.issued2019-11-01
dc.identifier.citationGuimerà, X. [et al.]. A minimally invasive microsensor specially designed for simultaneous dissolved oxygen and pH biofilm profiling. "Sensors", 1 Novembre 2019, vol. 19, núm. 21, p. 4747: 1-4747: 13.
dc.identifier.issn1424-8220
dc.identifier.urihttp://hdl.handle.net/2117/174820
dc.description.abstractA novel sensing device for simultaneous dissolved oxygen (DO) and pH monitoring specially designed for biofilm profiling is presented in this work. This device enabled the recording of instantaneous DO and pH dynamic profiles within biofilms, improving the tools available for the study and the characterization of biological systems. The microsensor consisted of two parallel arrays of microelectrodes. Microelectrodes used for DO sensing were bare gold electrodes, while microelectrodes used for pH sensing were platinum-based electrodes modified using electrodeposited iridium oxide. The device was fabricated with a polyimide (Kapton®) film of 127 µm as a substrate for minimizing the damage caused on the biofilm structure during its insertion. The electrodes were covered with a Nafion® layer to increase sensor stability and repeatability and to avoid electrode surface fouling. DO microelectrodes showed a linear response in the range 0-8 mg L-1, a detection limit of 0.05 mg L-1, and a sensitivity of 2.06 nA L mg-1. pH electrodes showed a linear super-Nernstian response (74.2 ± 0.7 mV/pH unit) in a wide pH range (pH 4-9). The multi-analyte sensor array was validated in a flat plate bioreactor where simultaneous and instantaneous pH and DO profiles within a sulfide oxidizing biofilm were recorded. The electrodes spatial resolution, the monitoring sensitivity, and the minimally invasive features exhibited by the proposed microsensor improved biofilm monitoring performance, enabling the quantification of mass transfer resistances and the assessment of biological activity.
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectÀrees temàtiques de la UPC::Enginyeria química::Biotecnologia
dc.subject.lcshBiofilms
dc.subject.lcshBioMEMS
dc.subject.lcshBiosensors
dc.subject.otherDO microsensor
dc.subject.otherpH microsensor
dc.subject.otherMulti-analyte sensor
dc.subject.otherMEMS technology
dc.subject.otherBiofilm profiling
dc.subject.otherBiofilm monitoring
dc.titleA minimally invasive microsensor specially designed for simultaneous dissolved oxygen and pH biofilm profiling
dc.typeArticle
dc.subject.lemacBiofilms
dc.subject.lemacBiosensors
dc.contributor.groupUniversitat Politècnica de Catalunya. BIOGAP - Grup de Tractament Biològic de Contaminants Gasosos i Olors
dc.identifier.doi10.3390/s19214747
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://www.mdpi.com/1424-8220/19/21/4747
dc.rights.accessOpen Access
local.identifier.drac26426413
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/MINECO/2PE/CTQ2015-69802-C2-2-R
local.citation.authorGuimerà, X.; Moya, A.; Dorado, A.D.; Illa, X.; Villa, R.; Gabriel, D.; Gamisans, X.; Gabriel, G.
local.citation.publicationNameSensors
local.citation.volume19
local.citation.number21
local.citation.startingPage4747: 1
local.citation.endingPage4747: 13


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Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution 4.0 Generic