Electrochemical sensor for bacterial metabolism based on the detection of NADH by Polythiophene nanoparticles
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Tipus de documentArticle
Data publicació2019-01-01
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Abstract
Composite i-PP/PEDOT films made of isotactic polypropylene (i-PP),
which is frequently used for the fabrication of implantable medical devices for internal use, and chemically synthesized poly(3,4 ethylendioxythiophene) (PEDOT) nanoparticles, which are electroactive and biocompatible, have been prepared and used to detect bacterial
metabolism. After chemical and morphological characterization, the properties (interfacial, mechanical, thermal, and electrochemical) and biocompatibility of i-PP/PEDOT have been examined. Besides, carbon screen-printed electrodes coated with i-PP/PEDOT have been found to detect the growth of Gram-positive and Gram-negative bacteria through the oxidation of nicotinamide adenine dinucleotide (NADH), which arises from the metabolism of bacteria (i.e., respiration). Thus, as outer bacterial membranes are permeable to cytosolic NADH, this metabolite has been found to be an appropriate target for the detection of bacterial
proliferation. In addition, the sensor does not respond to eukaryotic cells. This is because the major NADH pool in eukaryotic cells is located at the mitochondria and, therefore, the concentration of NADH in the medium is not high enough to be detected since the inner mitochondrial membrane is impermeable to NADH or NAD+.
CitacióMolina, B.G. [et al.]. Electrochemical sensor for bacterial metabolism based on the detection of NADH by Polythiophene nanoparticles. "Journal of chemical theory and computation", 1 Gener 2019, núm. 123, p. 22181-22190.
ISSN1549-9618
Versió de l'editorhttps://pubs.acs.org/doi/abs/10.1021/acs.jpcc.9b03982
Col·leccions
- IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies - Articles de revista [398]
- Doctorat en Polímers i Biopolímers - Articles de revista [152]
- PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables. - Articles de revista [181]
- Departament d'Enginyeria Química - Articles de revista [2.226]
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