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dc.contributor.authorZavradashvili, Nino
dc.contributor.authorSarisozen, Can
dc.contributor.authorTitvinidze, Giorgi
dc.contributor.authorOtinashvili, Giuli
dc.contributor.authorKantaria, Tengiz
dc.contributor.authorTugushi, David
dc.contributor.authorPuiggalí Bellalta, Jordi
dc.contributor.authorTorchilin, Vladimir P.
dc.contributor.authorKatsarava, Ramaz
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2019-11-25T18:12:04Z
dc.date.available2019-11-25T18:12:04Z
dc.date.issued2019-01-28
dc.identifier.citationZavradashvili, N. [et al.]. Library of cationic polymers composed of polyamines and arginine as gene transfection agents. "ACS omega", 28 Gener 2019, vol. 4, p. 2090-2101.
dc.identifier.issn2470-1343
dc.identifier.urihttp://hdl.handle.net/2117/173025
dc.description.abstractCombinatorially synthesized materials, especially cationic polymers (CPs), with gene transfection function hold great promise in nanotechnology. However, the main limitations of the existing CPs [such as polyethylenimine (PEI), poly-l-arginine, or polyamidoamine-based dendrimers] as gene transfection agents are high cytotoxicity in the physiological environment. We have developed novel CPs composed of polyamines—endogeneous tetraamine spermine (Spm) and synthetically made triamine N-(2-aminoethyl)-1,3-propanediamine (Apd) for incorporating sec-amino groups and imparting PEI-like structure to the CP backbones. Naturally occurring building blocks such as amino acid arginine (R) was also used for incorporating guanidine-groups into the CPs. The cytotoxicity of resulting CPs—polyureas (PUs) and polyamides such as polysuccinamides and R-attached polymalamides was evaluated using murine and human fibroblasts and carcinoma cell lines. The cell compatibility screening revealed that the CPs made of Apd are less cytotoxic compared to Spm-based analogues. From the novel polymer library, total of six polymers were further studied for oligonucleotide (pDNA) complexation and transfection abilities. Highly water-soluble CPs formed nano-sized polyplexes with pDNA at rather low CP/pDNA weight ratios and showed less cytotoxicity and higher transfection ability compared to widely used PEI as well as commercially available transfection agents. Furthermore, new CPs showed selective transfection activity toward certain cell lines (4T1, HeLa, NIH3T3, and CCD 27SK), which is important for their potential applications in gene therapy.
dc.format.extent12 p.
dc.language.isoeng
dc.publisherAmerican Chemical Society (ACS)
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria biomèdica
dc.subject.lcshPolymers
dc.subject.lcshTransfection
dc.subject.lcshGenetic engineering
dc.subject.lcshNanotechnology
dc.titleLibrary of cationic polymers composed of polyamines and arginine as gene transfection agents
dc.typeArticle
dc.subject.lemacPolímers
dc.subject.lemacEnginyeria genètica
dc.subject.lemacNanotecnologia
dc.contributor.groupUniversitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables.
dc.identifier.doi10.1021/acsomega.8b02977
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acsomega.8b02977
dc.rights.accessOpen Access
local.identifier.drac23941109
dc.description.versionPostprint (published version)
local.citation.authorZavradashvili, N.; Sarisozen, C.; Titvinidze, G.; Otinashvili, G.; Kantaria, T.; Tugushi, D.; Puiggali, J.; Torchilin; Katsarava, R.
local.citation.publicationNameACS omega
local.citation.volume4
local.citation.startingPage2090
local.citation.endingPage2101


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Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain