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dc.contributor.authorKarimi, Shadi
dc.contributor.authorMehrdel, Pouya
dc.contributor.authorFarré Lladós, Josep
dc.contributor.authorCasals Terré, Jasmina
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Mecànica
dc.date.accessioned2019-10-30T11:17:48Z
dc.date.available2020-09-01T00:28:07Z
dc.date.issued2019-09-03
dc.identifier.citationKarimi, S. [et al.]. A passive portable microfluidic blood-plasma separator for simultaneous determination of direct and indirect ABO/Rh blood typing. "Lab on a chip", 3 Setembre 2019, núm. 19, p. 3249-3260.
dc.identifier.issn1473-0197
dc.identifier.urihttp://hdl.handle.net/2117/171171
dc.description.abstractThe blood typing test is mandatory in any transfusion, organ transplant, and pregnancy situation. There is a lack of point-of-care (POC) blood typing that could perform both direct and indirect methods using a single droplet of whole blood. This study presents a new methodology combining a passive microfluidic blood–plasma separator (BPS) and a blood typing detector for the very first time, leading to a stand-alone microchip which is capable of determining the blood group from both direct and indirect methods simultaneously. The proposed design separates blood cells from plasma by applying hydrodynamic forces imposed on them, which overcomes the clogging issue and consequently maximizes the volume of the extracted plasma. An axial migration effect across the main channel is responsible for collecting the plasma in plasma collector channels. The BPS novel design approached 12% yield of plasma with 100% purity in approximately 10 minutes. The portable BPS was designed and fabricated to perform ABO/Rh blood tests based on the detection of agglutination in both antigens of RBCs (direct) and antibodies of plasma (indirect). The differences between agglutinated and non-agglutinated samples were distinguishable by the naked eye and also validated by particle analysis of microscopic pictures. The results of this passive BPS in ABO/Rh blood grouping verified the quality and quantity of the extracted plasma in practical applications
dc.format.extent12 p.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Enginyeria mecànica::Mecànica de fluids::Reologia
dc.subject.lcshMicrofluidics
dc.subject.lcshClinical chemishy
dc.subject.lcshDiagnosis, Laboratory
dc.subject.lcshBlood--Examination
dc.titleA passive portable microfluidic blood-plasma separator for simultaneous determination of direct and indirect ABO/Rh blood typing
dc.typeArticle
dc.subject.lemacMicrofluídica
dc.subject.lemacQuímica clínica
dc.subject.lemacDiagnòstic de laboratori
dc.subject.lemacSang -- Examen
dc.contributor.groupUniversitat Politècnica de Catalunya. MICROTECH LAB - Microtechnology for the Industry
dc.identifier.doi10.1039/c9lc00690g
dc.relation.publisherversionhttps://pubs.rsc.org/en/content/articlelanding/2019/lc/c9lc00690g#!divAbstract
dc.rights.accessOpen Access
local.identifier.drac25878450
dc.description.versionPostprint (author's final draft)
dc.relation.projectidinfo:eu-repo/grantAgreement/MINECO/2PE/CTQ2016-77936-R
dc.relation.projectidinfo:eu-repo/grantAgreement/MICINN/2PE/CTQ2017-84996-C2-1-R
local.citation.authorKarimi, S.; Mehrdel, P.; Farré-Lladós, J.; Casals-Terré, J.
local.citation.publicationNameLab on a chip
local.citation.number19
local.citation.startingPage3249
local.citation.endingPage3260


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