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GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment
dc.contributor.author | Francesko, Antonio |
dc.contributor.author | Soares da Costa, Diana |
dc.contributor.author | Lisboa, Patricia |
dc.contributor.author | Reis, Rui L. |
dc.contributor.author | Tzanov, Tzanko |
dc.contributor.author | Iva Hristova, Pashkuleva |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament d'Enginyeria Química |
dc.date.accessioned | 2012-12-04T12:03:01Z |
dc.date.created | 2012-04-20 |
dc.date.issued | 2012-04-20 |
dc.identifier.citation | Francesko, A. [et al.]. GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment. "Journal of materials chemistry", 20 Abril 2012, vol. 22, núm. 37, p. 19438-19446. |
dc.identifier.issn | 0959-9428 |
dc.identifier.uri | http://hdl.handle.net/2117/17072 |
dc.description.abstract | Multilayered polyelectrolyte coatings comprising thiolated chitosan (TC) and glycosaminoglycans (GAGs), chondroitin sulphate and hyaluronic acid, were built using a layer-by-layer approach. The surface activity of these coatings for binding and inhibition of enzymes related to chronic inflammation, such as collagenase and myeloperoxidase, was assessed. The build-up of five bi-layers of TC/GAGs onto gold surfaces was monitored in situ by QCM-D. All experimental groups showed exponential growth of the coatings controlled by the degree of chitosan thiolation and the molecular weight of the GAGs. The degree of chitosan modification was also the key parameter influencing the enzyme activity: increasing the thiols content led to more efficient myeloperoxidase inhibition and was inversely proportional to the adsorption of collagenase. Enhanced fibroblast attachment and proliferation were observed when the multilayered polyelectrolyte constructs terminated with GAGs. The possibility to control either the activity of major wound enzymes by the thiolation degree of the coating or the cell adhesion and proliferation by proper selection of the ultimate layer makes these materials potentially useful in chronic wounds treatment and dermal tissue regeneration. |
dc.format.extent | 9 p. |
dc.language.iso | eng |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Enginyeria química |
dc.subject.lcsh | Biomedical materials. |
dc.title | GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment |
dc.type | Article |
dc.subject.lemac | Biomaterials |
dc.contributor.group | Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial |
dc.identifier.doi | 10.1039/C2JM31051A |
dc.description.peerreviewed | Peer Reviewed |
dc.rights.access | Restricted access - publisher's policy |
local.identifier.drac | 10288094 |
dc.description.version | Postprint (published version) |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/FP7/229292/EU/Find and Bind: Mastering sweet cell-instructive biosystems by copycat nano-interaction of cells with natural surfaces for biotechnological applications/FIND AND BIND |
dc.date.lift | 10000-01-01 |
local.citation.author | Francesko, A.; Soares da Costa, D.; Lisboa, P.; Reis, R.; Tzanov, T.; Pashkuleva, I.H. |
local.citation.publicationName | Journal of materials chemistry |
local.citation.volume | 22 |
local.citation.number | 37 |
local.citation.startingPage | 19438 |
local.citation.endingPage | 19446 |
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