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dc.contributor.authorFrancesko, Antonio
dc.contributor.authorSoares da Costa, Diana
dc.contributor.authorLisboa, Patricia
dc.contributor.authorReis, Rui L.
dc.contributor.authorTzanov, Tzanko
dc.contributor.authorIva Hristova, Pashkuleva
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2012-12-04T12:03:01Z
dc.date.created2012-04-20
dc.date.issued2012-04-20
dc.identifier.citationFrancesko, A. [et al.]. GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment. "Journal of materials chemistry", 20 Abril 2012, vol. 22, núm. 37, p. 19438-19446.
dc.identifier.issn0959-9428
dc.identifier.urihttp://hdl.handle.net/2117/17072
dc.description.abstractMultilayered polyelectrolyte coatings comprising thiolated chitosan (TC) and glycosaminoglycans (GAGs), chondroitin sulphate and hyaluronic acid, were built using a layer-by-layer approach. The surface activity of these coatings for binding and inhibition of enzymes related to chronic inflammation, such as collagenase and myeloperoxidase, was assessed. The build-up of five bi-layers of TC/GAGs onto gold surfaces was monitored in situ by QCM-D. All experimental groups showed exponential growth of the coatings controlled by the degree of chitosan thiolation and the molecular weight of the GAGs. The degree of chitosan modification was also the key parameter influencing the enzyme activity: increasing the thiols content led to more efficient myeloperoxidase inhibition and was inversely proportional to the adsorption of collagenase. Enhanced fibroblast attachment and proliferation were observed when the multilayered polyelectrolyte constructs terminated with GAGs. The possibility to control either the activity of major wound enzymes by the thiolation degree of the coating or the cell adhesion and proliferation by proper selection of the ultimate layer makes these materials potentially useful in chronic wounds treatment and dermal tissue regeneration.
dc.format.extent9 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshBiomedical materials.
dc.titleGAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment
dc.typeArticle
dc.subject.lemacBiomaterials
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.1039/C2JM31051A
dc.description.peerreviewedPeer Reviewed
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac10288094
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/229292/EU/Find and Bind: Mastering sweet cell-instructive biosystems by copycat nano-interaction of cells with natural surfaces for biotechnological applications/FIND AND BIND
dc.date.lift10000-01-01
local.citation.authorFrancesko, A.; Soares da Costa, D.; Lisboa, P.; Reis, R.; Tzanov, T.; Pashkuleva, I.H.
local.citation.publicationNameJournal of materials chemistry
local.citation.volume22
local.citation.number37
local.citation.startingPage19438
local.citation.endingPage19446


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