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dc.contributor.authorCarro, Juan
dc.contributor.authorGonzález-Benjumea, Alejandro
dc.contributor.authorFernández-Fueyo, Elena
dc.contributor.authorAranda, Carmen
dc.contributor.authorGuallar, Victor
dc.contributor.authorGutiérrez, Ana
dc.contributor.authorMartínez, Angel T.
dc.contributor.otherBarcelona Supercomputing Center
dc.date.accessioned2019-07-19T13:21:49Z
dc.date.available2019-07-19T13:21:49Z
dc.date.issued2019-06-14
dc.identifier.citationCarro, J. [et al.]. Modulating Fatty Acid Epoxidation vs Hydroxylation in a Fungal Peroxygenase. "ACS Catalysis", 14 Juny 2019, vol. 9, núm. 7, p. 6234-6242.
dc.identifier.issn2155-5435
dc.identifier.urihttp://hdl.handle.net/2117/166459
dc.description.abstractUnspecific peroxygenases (UPOs) are fungal secreted counterparts of the cytochrome P450 monooxygenases present in most living cells. Both enzyme types share the ability to perform selective oxygenation reactions. Moreover, the Marasmius rotula UPO (MroUPO) catalyzes reactions of interest compared with the previously described UPOs, including formation of reactive epoxy fatty acids. To investigate substrate epoxidation, the most frequent positions of oleic acid at the MroUPO heme channel were predicted using binding and molecular dynamics simulations. Then, mutations in neighbor residues were designed aiming at modulating the enzyme epoxidation vs hydroxylation ratio. Both the native (wild-type recombinant) MroUPO and the mutated variants were expressed in Escherichia coli as active enzymes, and their action on oleic and other fatty acids was investigated by gas chromatography–mass spectrometry in combination with kinetic analyses. Interestingly, a small modification of the channel shape in the I153T variant increased the ratio between epoxidized oleic acid and its additionally hydroxylated derivatives. A fully opposite effect was attained with the double I153F/S156F variant that completely abolished the ability of the MroUPO to epoxidize oleic acid (while no activity was detected for the I153V variant). The rationale for these results was revealed by the substrate positioning in the above computational simulations, which predict a shorter distance between the oleic acid double bond and the oxygen atom of the peroxide-activated heme (compound I) in the I153T variant than in the native enzyme, promoting epoxidation. In contrast, the I153F/S156F double mutation fully prevents the approach of oleic acid in the bent conformation required for double-bond epoxidation, although its (sub)terminal hydroxylation was predicted and experimentally confirmed. The I153T mutation also increased the UPO selectivity on polyunsaturated fatty acid epoxidation, strongly reducing the ratio between simple epoxides and their hydroxylated derivatives, with respect to the native UPO.
dc.description.sponsorshipThis work has been funded by the BBI-JU (www.bbi-europe.eu) project SusBind (on “Sustainable biobinders”; H2020-BBI-JTI-2017-792063; https://susbind.eu), together with the CTQ2016-79138-R and BIO2017-86559-R (GENOBIOREF) projects of the Spanish Ministry of Economy, Industry and Competitiveness, cofinanced by FEDER funds. The authors thank Andrés Olmedo (IRNAS, Sevilla) for his useful comments.
dc.format.extent9 p.
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.subjectÀrees temàtiques de la UPC::Ciències de la salut
dc.subject.lcshEnzyme regulation
dc.subject.otherUnspecific peroxygenase
dc.subject.otherMarasmius rotula
dc.subject.otherUnsaturated fatty acids
dc.subject.otherEpoxidation
dc.subject.otherHydroxylation
dc.subject.otherComputational simulations
dc.subject.otherPELE software
dc.subject.otherGas chromatography−mass spectrometry
dc.titleModulating Fatty Acid Epoxidation vs Hydroxylation in a Fungal Peroxygenase
dc.typeArticle
dc.subject.lemacEnzims -- Anàlisi
dc.identifier.doi10.1021/acscatal.9b01454
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://pubs.acs.org/doi/full/10.1021/acscatal.9b01454
dc.rights.accessOpen Access
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/H2020/792063/EU/Development and pilot production of SUStainable bio BINDer systems for wood based panels/SusBind
dc.relation.projectidinfo:eu-repo/grantAgreement/MINECO/PE2013-2016/CTQ2016-79138-R
dc.relation.projectidinfo:eu-repo/grantAgreement/MINECO/PE2013-2016/BIO2017-86559-R
local.citation.publicationNameACS Catalysis
local.citation.volume9
local.citation.number7
local.citation.startingPage6234
local.citation.endingPage6242


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