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dc.contributor.authorLoza Álvarez, Pablo
dc.contributor.authorArtigas García, David
dc.contributor.authorSantos, Susana I.C.O.
dc.contributor.authorAviles Espinosa, Rodrigo
dc.contributor.authorFilippidis, George
dc.contributor.authorHamilton, Craig
dc.contributor.authorMalcolm, Graeme
dc.contributor.authorWeingarten, Kurt J.
dc.contributor.authorSüdmeyer, Thomas
dc.contributor.authorBarbarin, Yohan
dc.contributor.authorKeller, Ursula
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions
dc.date.accessioned2012-08-29T12:11:18Z
dc.date.created2011-03-01
dc.date.issued2011-03-01
dc.identifier.citationAviles, R. [et al.]. Compact ultrafast semiconductor disk laser: targeting GFP based nonlinear applications in living organisms. "BIOMEDICAL OPTICS EXPRESS", 01 Març 2011, vol. 2, núm. 4, p. 739-747.
dc.identifier.issn2156-7085
dc.identifier.urihttp://hdl.handle.net/2117/16403
dc.description.abstractWe present a portable ultrafast Semiconductor Disk Laser (SDL) (or vertical extended cavity surface emitting laser—VECSELs), to be used for nonlinear microscopy. The SDL is modelocked using a quantum-dot semiconductor saturable absorber mirror (SESAM), delivering an average output power of 287 mW, with 1.5 ps pulses at 500 MHz and a central wavelength of 965 nm. Specifically, despite the fact of having long pulses and high repetition rates, we demonstrate the potential of this laser for Two-Photon Excited Fluorescence (TPEF) imaging of in vivo Caenorhabditis elegans (C. elegans) expressing Green Fluorescent Protein (GFP) in a set of neuronal processes and cell bodies. Efficient TPEF imaging is achieved due to the fact that this wavelength matches the peak of the two-photon action cross section of this widely used fluorescent marker. The SDL extended versatility is shown by presenting Second Harmonic Generation images of pharynx, uterus, body wall muscles and its potential to be used to excite other different commercial dyes. Importantly this non-expensive, turn-key, compact laser system could be used as a platform to develop portable nonlinear bio-imaging devices.
dc.format.extent9 p.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Física
dc.subject.lcshNonlinear optics
dc.subject.otherSATURABLE ABSORBER
dc.subject.other2-PHOTON EXCITATION
dc.subject.otherFLUORESCENCE MICROSCOPY
dc.subject.otherMULTIPHOTON MICROSCOPY
dc.subject.otherSCANNING MICROSCOPY
dc.subject.otherPULSE SOURCE
dc.subject.otherFEMTOSECOND
dc.subject.otherDIODE
dc.subject.otherGENERATION
dc.subject.otherNEURONS
dc.titleCompact ultrafast semiconductor disk laser: targeting GFP based nonlinear applications in living organisms
dc.typeArticle
dc.subject.lemacÒptica no lineal
dc.contributor.groupUniversitat Politècnica de Catalunya. FOTONICA - Grup de Recerca de Fotònica
dc.identifier.doi10.1364/BOE.2.000739
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072117/
dc.rights.accessRestricted access - publisher's policy
drac.iddocument9862585
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/217997/EU/Spatio-Temporal Engineering of Light. Ultimate Multiphoton Microscopy/STELUM
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/224338/EU/COMPACT ULTRAFAST LASER SOURCES BASED ON NOVEL QUANTUM DOT STRUCTURES/FAST-DOT
dc.date.lift10000-01-01
upcommons.citation.authorAviles, R.; Filippidis, G.; Hamilton, C.; Malcolm, G.; Weingarten, K.; Südmeyer, T.; Barbarin, Y.; Keller, U.; Santos, S.; Artigas, D.; Loza, P.
upcommons.citation.publishedtrue
upcommons.citation.publicationNameBIOMEDICAL OPTICS EXPRESS
upcommons.citation.volume2
upcommons.citation.number4
upcommons.citation.startingPage739
upcommons.citation.endingPage747
dc.identifier.pmid21483599


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