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dc.contributor.authorAviles Espinosa, Rodrigo
dc.contributor.authorAndilla, Jordi
dc.contributor.authorPorcar-Guezenec, Rafael
dc.contributor.authorOlarte, Omar E.
dc.contributor.authorNieto, Marta
dc.contributor.authorLevecq, Xavier
dc.contributor.authorArtigas García, David
dc.contributor.authorLoza Álvarez, Pablo
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions
dc.date.accessioned2012-08-29T11:59:45Z
dc.date.available2012-08-29T11:59:45Z
dc.date.created2011-11-01
dc.date.issued2011-11-01
dc.identifier.citationAviles, R. [et al.]. Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy. "BIOMEDICAL OPTICS EXPRESS", 01 Novembre 2011, vol. 2, núm. 11, p. 3135-3149.
dc.identifier.issn2156-7085
dc.identifier.urihttp://hdl.handle.net/2117/16402
dc.description.abstractWe demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear guide-star (NL-GS). The wavefront emitted from the NL-GS can then be recorded using a Shack-Hartmann sensor. Compensation of the recorded sample aberrations is performed by the deformable mirror in a single-step. This technique is applied to fixed and in vivo biological samples, showing, in some cases, more than one order of magnitude improvement in the total collected signal intensity.
dc.format.extent15 p.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Física
dc.subject.lcshNonlinear optics
dc.subject.otherAdaptative optics
dc.subject.otherMultiphoton microscopy
dc.subject.otherSpherical-aberration
dc.subject.otherDeep-Tissue
dc.titleMeasurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy
dc.typeArticle
dc.subject.lemacÒptica no lineal
dc.contributor.groupUniversitat Politècnica de Catalunya. FOTONICA - Grup de Recerca de Fotònica
dc.identifier.doi10.1364/BOE.2.003135
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://www.ncbi.nlm.nih.gov/pubmed/22076274
dc.rights.accessOpen Access
local.identifier.drac9852272
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/217997/EU/Spatio-Temporal Engineering of Light. Ultimate Multiphoton Microscopy/STELUM
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/224014/EU/Network of Excellence for Biophotonics/PHOTONICS4LIFE
local.citation.authorAviles, R.; Andilla, J.; Porcar-Guezenec, R.; Olarte, O.; Nieto, M.; Levecq, X.; Artigas, D.; Loza, P.
local.citation.publicationNameBIOMEDICAL OPTICS EXPRESS
local.citation.volume2
local.citation.number11
local.citation.startingPage3135
local.citation.endingPage3149
dc.identifier.pmid22076274


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