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dc.contributor.authorCardona, Pere-Joan
dc.contributor.authorCáceres, Neus
dc.contributor.authorLlopis Fuste, Isaac
dc.contributor.authorMarzo, Elena
dc.contributor.authorPrats Soler, Clara
dc.contributor.authorVilaplana, Cristina
dc.contributor.authorGarcía de Viedma, Darío
dc.contributor.authorSamper, Sofia
dc.contributor.authorLópez Codina, Daniel
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Física i Enginyeria Nuclear
dc.date.accessioned2012-07-11T12:56:31Z
dc.date.available2012-07-11T12:56:31Z
dc.date.created2012-01-03
dc.date.issued2012-01-03
dc.identifier.citationCáceres, N. [et al.]. Low dose aerosol fitness at the innate phase of murine infection better predicts virulence amongst clinical strains of mycobacterium tuberculosis. "PLoS One", 03 Gener 2012, vol. 7, núm. 1.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/2117/16232
dc.description.abstractBackground: Evaluation of a quick and easy model to determine the intrinsic ability of clinical strains to generate active TB has been set by assuming that this is linked to the fitness of Mycobacterium tuberculosis strain at the innate phase of the infection. Thus, the higher the bacillary load, the greater the possibility of inducting liquefaction, and thus active TB, once the adaptive response is set. Methodology/Principal Findings: The virulence of seven clinical Mycobacterium tuberculosis strains isolated in Spain was tested by determining the bacillary concentration in the spleen and lung of mice at weeks 0, 1 and 2 after intravenous (IV) inoculation of 104 CFU, and by determining the growth in vitro until the stationary phase had been reached. Cord distribution automated analysis showed two clear patterns related to the high and low fitness in the lung after IV infection. This pattern was not seen in the in vitro fitness tests, which clearly favored the reference strain (H37Rv). Subsequent determination using a more physiological low-dose aerosol (AER) inoculation with 102 CFU showed a third pattern in which the three best values coincided with the highest dissemination capacity according to epidemiological data. Conclusions/Significance: The fitness obtained after low dose aerosol administration in the presence of the innate immune response is the most predictive factor for determining the virulence of clinical strains. This gives support to a mechanism of the induction of active TB derived from the dynamic hypothesis of latent tuberculosis infection.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Ciències de la salut::Medicina::Medicina interna
dc.subject.lcshTuberculosis -- Research
dc.titleLow dose aerosol fitness at the innate phase of murine infection better predicts virulence amongst clinical strains of mycobacterium tuberculosis
dc.typeArticle
dc.subject.lemacTuberculosi -- Recerca
dc.contributor.groupUniversitat Politècnica de Catalunya. SC-SIMBIO - Sistemes complexos. Simulació discreta de materials i de sistemes biològics
dc.identifier.doi10.1371/journal.pone.0029010
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
local.identifier.drac8946747
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/200999/EU/LATENT TUBERCULOSIS: New tools for the detection and clearance of dormant Mycobacterium tuberculosis./STOPLATENT-TB
local.citation.authorCáceres, N.; Llopis, I.; Marzo, E.; Prats, C.; Vilaplana, C.; García de Viedma, D.; Samper, S.; Lopez, D.; Cardona, P.
local.citation.publicationNamePLoS One
local.citation.volume7
local.citation.number1
local.citation.startingPagee29010
dc.identifier.pmid22235258


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