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dc.contributor.authorPegueroles Neyra, Marta
dc.contributor.authorAguirre Cano, Aitor
dc.contributor.authorEngel López, Elisabeth
dc.contributor.authorPavon-Djavid, Graciela
dc.contributor.authorGil Mur, Francisco Javier
dc.contributor.authorPlanell Estany, Josep Anton
dc.contributor.authorMigonney, Veronique
dc.contributor.authorAparicio Bádenas, Conrado José
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.contributor.otherInstitut de Bioenginyeria de Catalunya
dc.date.accessioned2012-01-17T11:42:09Z
dc.date.available2012-01-17T11:42:09Z
dc.date.created2011-03-02
dc.date.issued2011-03-02
dc.identifier.citationPegueroles, Marta [et al.]. Effect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titanium: a gene expression study using real-time RT-PCR.. "Journal of materials science. Materials in medicine", 02 Març 2011, vol. 22, núm. 3, p. 617-627.
dc.identifier.issn0957-4530
dc.identifier.urihttp://hdl.handle.net/2117/14596
dc.description.abstractBiomaterial surface properties, via alterations in the adsorbed protein layer, and the presence of specific functional groups can influence integrin binding specificity, thereby modulating cell adhesion and differentiation processes. The adsorption of fibronectin, a protein directly involved in osteoblast adhesion to the extracellular matrix, has been related to different physical and chemical properties of biomaterial surfaces. This study used blasting particles of different sizes and chemical compositions to evaluate the response of MG63 osteoblast-like cells on smooth and blasted titanium surfaces, with and without fibronectin coatings, by means of real-time reverse transcription-polymerase chain reaction (qRT-PCR) assays. This response included (a) expression of the a5, av and a3 integrin subunits, which can bind to fibronectin through the RGD binding site, and (b) expression of alkaline phosphatase (ALP) and osteocalcin (OC) as cell-differentiation markers. ALP activity and synthesis of OC were also tested. Cells on SiC-blasted Ti surfaces expressed higher amounts of the a5 mRNA gene than cells on Al2O3-blasted Ti surfaces. This may be related to the fact that SiC-blasted surfaces adsorbed higher amounts of fibronectin due to their higher surface free energy and therefore provided a higher number of specific cell-binding sites. Fn-coated Tisurfaces decreased a5 mRNA gene expression, by favoring the formation of other integrins involved in adhesion over a5b1. The changes in a5 mRNA expression induced by the presence of fibronectin coatings may moreover influence the osteoblast differentiation pathway, as fibronectin coatings on Ti surfaces also decreased both ALP mRNA expression and ALP activity after 14 and 21 days of cell culture.
dc.format.extent11 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshBiomaterials
dc.titleEffect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titanium: a gene expression study using real-time RT-PCR.
dc.typeArticle
dc.subject.lemacBiomaterials
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1007/s10856-011-4229-3
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://www.springerlink.com/content/g24501773800tq31/
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac9411615
dc.description.versionPostprint (published version)
local.citation.authorPegueroles, Marta; Aguirre, A.; Engel, E.; Pavon-Djavid, Graciela; Gil, F.J.; Planell, J.; Migonney, Veronique; Aparicio, C.
local.citation.publicationNameJournal of materials science. Materials in medicine
local.citation.volume22
local.citation.number3
local.citation.startingPage617
local.citation.endingPage627


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