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dc.contributor.authorKoch, M.A.
dc.contributor.authorVrij, E.J.
dc.contributor.authorEngel López, Elisabeth
dc.contributor.authorPlanell Estany, Josep Anton
dc.contributor.authorLacroix, Damien Jerome
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.contributor.otherInstitut de Bioenginyeria de Catalunya
dc.date.accessioned2012-01-17T11:23:55Z
dc.date.available2012-01-17T11:23:55Z
dc.date.created2010-12-17
dc.date.issued2010-12-17
dc.identifier.citationKoch, M. [et al.]. Perfusion cell seeding on large porous PLA/calcium phosphate composite scaffolds in a perfusion bioreactor system under varying perfusion parameters.. "Journal of biomedical materials research. Part A", 17 Desembre 2010, vol. 95, núm. 4, p. 1011-1018.
dc.identifier.issn1549-3296
dc.identifier.urihttp://hdl.handle.net/2117/14595
dc.description.abstractA promising approach to bone tissue engineering lies in the use of perfusion bioreactors where cells are seeded and cultured on scaffolds under conditions of enhanced nutrient supply and removal of metabolic products. Fluid flow alterations can stimulate cell activity, making the engineering of tissue more efficient. Most bioreactor systems are used to culture cells on thin scaffold discs. In clinical use, however, bone substitutes of large dimensions are needed. In this study, MG63 osteoblast-like cells were seeded on large porous PLA/glass scaffolds with a custom developed perfusion bioreactor system. Cells were seeded by oscillating perfusion of cell suspension through the scaffolds. Applicable perfusion parameters for successful cell seeding were determined by varying fluid flow velocity and perfusion cycle number. After perfusion, cell seeding, the cell distribution, and cell seeding efficiency were determined. A fluid flow velocity of 5 mm/s had to be exceeded to achieve a uniform cell distribution throughout the scaffold interior. Cell seeding efficiencies of up to 50% were achieved. Results suggested that perfusion cycle number influenced cell seeding efficiency rather than fluid flow velocities. The cell seeding conducted is a promising basis for further long term cell culture studies in large porous scaffolds.
dc.format.extent8 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshBioreactors
dc.subject.lcshTissue scaffolds
dc.subject.lcshBones
dc.titlePerfusion cell seeding on large porous PLA/calcium phosphate composite scaffolds in a perfusion bioreactor system under varying perfusion parameters.
dc.typeArticle
dc.subject.lemacBioreactors
dc.subject.lemacEnginyeria de teixits
dc.subject.lemacOssos
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1002/jbm.a.32927
dc.description.peerreviewedPeer Reviewed
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac9411671
dc.description.versionPostprint (published version)
local.citation.authorKoch, M.; Vrij, E.; Engel, E.; Planell, J.; Lacroix, D.
local.citation.publicationNameJournal of biomedical materials research. Part A
local.citation.volume95
local.citation.number4
local.citation.startingPage1011
local.citation.endingPage1018


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