Proteolytic enzymes in detergents: evidence of their presence through activity measurements based on electrophoresis
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hdl:2117/14514
Tipus de documentArticle
Data publicació2011
Condicions d'accésAccés obert
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Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
Abstract
This laboratory exercise uses a problem-based approach to expose
students to some basic concepts relating to proteins and enzymes. One of the main
applications of enzymes at the industrial level is their use in the detergent market. The
students examine a detergent sample to ascertain whether proteolytic enzymes are a
component and, if so,which colored detergent granules contain them.A4-hour standard
exercise is described that can be easily modified to study a number of conditions that
affect enzymatic activity. The students also become familiar with polyacrylamide gel
electrophoresis as it is used to visualize the products of enzyme activity.
CitacióSaperas, N.; Fonfria, E. Proteolytic enzymes in detergents: evidence of their presence through activity measurements based on electrophoresis. "Journal of chemical education", 2011, vol. 88, núm. 12, p. 1702-1706.
ISSN0021-9584
Versió de l'editorhttp://pubs.acs.org/doi/abs/10.1021/ed2001285
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