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dc.contributor.authorFittipaldi Gustavino, Mariana
dc.contributor.authorPino Rodriguez, Nancy J.
dc.contributor.authorAdrados Ruiz, Barbara
dc.contributor.authorAgustí Adalid, Gemma
dc.contributor.authorPeñuela, Gustavo
dc.contributor.authorMorató Farreras, Jordi
dc.contributor.authorCodony Iglesias, Francesc
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Òptica i Optometria
dc.identifier.citationFittipaldi, M. [et al.]. Discrimination of viable acanthamoeba castellani trophozoites and cysts by propidium monoazide real-time polymerase chain reaction. "Journal of eukaryotic microbiology", Agost 2011, vol. 58, núm. 4, p. 359-364.
dc.description.abstractEven though the advent of quantitative polymerase chain reaction (PCR) has improved the detection of pathogen microorganisms in most of areas of microbiology, a serious limitation of this method may arise from the inability to discriminate between viable and nonviable pathogens. To overcome it, the use of real-time PCR and selective nucleic acid intercalating dyes like propidium monoazide (PMA) have been effectively evaluated for different microorganisms. To assess whether PMA pretreatment can inhibit PCR amplification of nonviable amoeba DNA, Acanthamoeba castellani survival was measured using cell culture and real-time PCR with and without PMA pretreatment. Autoclave and contact lens disinfecting solutions were used to inactivate amoebae. After these inactivation treatments, the results indicated that the PMA pretreatment approach is appropriate for differentiating viable A. castellani, both trophozoites and cysts. Therefore, the PMA-PCR approach could be useful as a rapid and sensitive analytical tool for monitoring treatment and disease control, assessing effective disinfection treatments, and for a more reliable understanding of the factors that contribute to the interaction amoeba– pathogenic bacteria.
dc.format.extent6 p.
dc.subjectÀrees temàtiques de la UPC::Ciències de la visió::Biologia ocular
dc.subject.lcshContact lens solutions
dc.titleDiscrimination of viable acanthamoeba castellani trophozoites and cysts by propidium monoazide real-time polymerase chain reaction
dc.subject.lemacLents de contacte -- Biomicroscòpia
dc.contributor.groupUniversitat Politècnica de Catalunya. TMAS (T+) - Toxicologia i Microbiologia Ambiental i Sanitària
dc.description.peerreviewedPeer Reviewed
dc.rights.accessRestricted access - publisher's policy
dc.description.versionPostprint (published version)
local.citation.authorFittipaldi, M.; Pino, N.; Adrados, B.; Agusti, G.; Peñuela, G.; Morató, J.; Codony, F.
local.citation.publicationNameJournal of eukaryotic microbiology

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