Ir al contenido (pulsa Retorno)

Universitat Politècnica de Catalunya

    • Català
    • Castellano
    • English
    • LoginRegisterLog in (no UPC users)
  • mailContact Us
  • world English 
    • Català
    • Castellano
    • English
  • userLogin   
      LoginRegisterLog in (no UPC users)

UPCommons. Global access to UPC knowledge

58.917 UPC E-Prints
You are here:
View Item 
  •   DSpace Home
  • E-prints
  • Grups de recerca
  • GBMI - Grup de Biotecnologia Molecular i Industrial
  • Articles de revista
  • View Item
  •   DSpace Home
  • E-prints
  • Grups de recerca
  • GBMI - Grup de Biotecnologia Molecular i Industrial
  • Articles de revista
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Hydrophobic amino acids at the cytoplasmic ends of helices 3 and 6 of rhodopsin conjointly modulate transducin activation

Thumbnail
View/Open
Hydrophobic amino acids.pdf (768,6Kb) (Restricted access)   Request copy 

Què és aquest botó?

Aquest botó permet demanar una còpia d'un document restringit a l'autor. Es mostra quan:

  • Disposem del correu electrònic de l'autor
  • El document té una mida inferior a 20 Mb
  • Es tracta d'un document d'accés restringit per decisió de l'autor o d'un document d'accés restringit per política de l'editorial
Share:
 
 
10.1016/j.abb.2010.11.019
 
  View Usage Statistics
Cita com:
hdl:2117/13237

Show full item record
Bosch Presegué, Laia
Iarriccio Silva, Laura
Aguila Cerda, Mónica
Toledo, Darwin
Ramon Portés, EvaMés informacióMés informacióMés informació
Cordomí Montoya, Arnau
Garriga Solé, PereMés informacióMés informacióMés informació
Document typeArticle
Defense date2011-02-15
Rights accessRestricted access - publisher's policy
All rights reserved. This work is protected by the corresponding intellectual and industrial property rights. Without prejudice to any existing legal exemptions, reproduction, distribution, public communication or transformation of this work are prohibited without permission of the copyright holder
Abstract
Rhodopsin is the visual photoreceptor responsible for dim light vision. This receptor is located in the rod cell of the retina and is a prototypical member of the G-protein-coupled receptor superfamily. The structural details underlying the molecular recognition event in transducin activation by photoactivated rhodopsin are of key interest to unravel the molecular mechanism of signal transduction in the retina. We constructed and expressed rhodopsin mutants in the second and third cytoplasmic domains of rhodopsin –where the natural amino acids were substituted by the human M3 acetylcholine muscarinic receptor homologous residues– in order to determine their potential involvement in G-protein recognition. These mutants showed normal chromophore formation and a similar photobleaching behavior than WT rhodopsin, but decreased thermal stability in the dark state. The single mutant V1383.53 and the multiple mutant containing V2275.62 and a combination of mutations at the cytoplasmic end of transmembrane helix 6 caused a reduction in transducin activation upon rhodopsin photoactivation. Furthermore, combination of mutants at the second and third cytoplasmic domains revealed a cooperative role, and partially restored transducin activation. The results indicate that hydrophobic interactions by V1383.53, V2275.62, V2506.33, V2546.37 and I2556.38 are critical for receptor activation and/or efficient rhodopsin–transducin interaction.
CitationBosch [et al.]. Hydrophobic amino acids at the cytoplasmic ends of helices 3 and 6 of rhodopsin conjointly modulate transducin activation. "Archives of biochemistry and biophysics", 15 Febrer 2011, vol. 506, núm. 2, p. 142-149. 
URIhttp://hdl.handle.net/2117/13237
DOI10.1016/j.abb.2010.11.019
ISSN0003-9861
Collections
  • GBMI - Grup de Biotecnologia Molecular i Industrial - Articles de revista [170]
  • Departament d'Enginyeria Química - Articles de revista [1.992]
Share:
 
  View Usage Statistics

Show full item record

FilesDescriptionSizeFormatView
Hydrophobic amino acids.pdfBlocked768,6KbPDFRestricted access

Browse

This CollectionBy Issue DateAuthorsOther contributionsTitlesSubjectsThis repositoryCommunities & CollectionsBy Issue DateAuthorsOther contributionsTitlesSubjects

© UPC Obrir en finestra nova . Servei de Biblioteques, Publicacions i Arxius

info.biblioteques@upc.edu

  • About This Repository
  • Contact Us
  • Send Feedback
  • Inici de la pàgina