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Electrical evaluation of bacterial virulence factors using nanopores
dc.contributor.author | Escosura Muñiz, Alfredo de la |
dc.contributor.author | Ivanova, Kristina Dimitrova |
dc.contributor.author | Tzanov, Tzanko |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament d'Enginyeria Química |
dc.date.accessioned | 2019-04-03T11:03:34Z |
dc.date.available | 2020-03-19T01:25:59Z |
dc.date.issued | 2019-03-19 |
dc.identifier.citation | De La Escosura, A.; Ivanova, K.; Tzanov, T. Electrical evaluation of bacterial virulence factors using nanopores. "ACS Applied materials and interfaces", 19 Març 2019, vol.11, núm. 14, p.13140-131146 |
dc.identifier.issn | 1944-8252 |
dc.identifier.uri | http://hdl.handle.net/2117/131193 |
dc.description.abstract | In this work, we propose a novel methodology for electrical monitoring using nanoporous alumina membranes of virulence factors secreted by bacterial pathogens. Bacterial hyaluronidase (HYAL), which is produced by a number of invasive Gram-positive bacteria, is selected as a model compound to prove the concept. Our electrochemical setup takes advantage of the flat surface of indium tin oxide/poly(ethylene terephthalate) (ITO/PET) electrodes for their assembly with the nanoporous membrane. The proposed analytical method, based on the electrical monitoring of the steric/electrostatic nanochannels blocked upon formation of an antibody–HYAL immunocomplex, reached detection limits as low as 64 UI/mL (17.3 U/mg) HYAL. The inert surface of the ITO/PET electrodes together with the anti-biofilm properties of the 20 nm pore-sized alumina membranes allows for culturing the bacteria, capturing the secreted enzymes inside the nanochannels, and removing the cells before the electrochemical measurement. Secreted HYAL at levels of 1000 UI/mL (270 U/mg) are estimated in Gram-positive Staphylococcus aureus cultures, whereas low levels are detected for Gram-negative Pseudomonas aeruginosa (used as a negative control). Finally, HYAL secretion inhibition by RNAIII-inhibiting peptide (YSPWTNF-NH2) is also monitored, opening the way for further applications of the developed monitoring system for evaluation of the antivirulence potential of different compounds. This label-free method is rapid and cheap, avoiding the use of the time-consuming sandwich assays. We envisage future applications for monitoring of bacterial virulence/invasion as well as for testing of novel antimicrobial/antivirulence agents. |
dc.format.extent | 7 p. |
dc.language.iso | eng |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Enginyeria química |
dc.subject.lcsh | Biomedical engineering |
dc.subject.lcsh | Biotechnology |
dc.subject.other | bacteria |
dc.subject.other | electrochemical detection |
dc.subject.other | hyaluronidase |
dc.subject.other | nanochannels |
dc.subject.other | nanopores |
dc.subject.other | virulence factor |
dc.title | Electrical evaluation of bacterial virulence factors using nanopores |
dc.type | Article |
dc.subject.lemac | Enginyeria biomèdica |
dc.subject.lemac | Biotecnologia |
dc.subject.lemac | Nanoporus |
dc.contributor.group | Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial |
dc.identifier.doi | 10.1021/acsami.9b02382 |
dc.description.peerreviewed | Peer Reviewed |
dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/acsami.9b02382 |
dc.rights.access | Open Access |
local.identifier.drac | 24017907 |
dc.description.version | Postprint (published version) |
dc.relation.projectid | info:eu-repo/grantAgreement/MINECO/1PE/PCIN-2016-134 |
local.citation.author | De La Escosura, A.; Ivanova, K.; Tzanov, T. |
local.citation.publicationName | ACS Applied materials and interfaces |
local.citation.startingPage | 1 |
local.citation.endingPage | 7 |
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