Development of a novel microsensor for the study of oxygen profiles in biofilms
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Inclou dades d'ús des de 2022
Cita com:
hdl:2117/130649
Tipus de documentComunicació de congrés
Data publicació2013
Condicions d'accésAccés obert
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Abstract
Understanding of the processes taking place inside biofilms is a key parameter to progress in the optimization of biofiltration technologies. This study was conducted with the aim of developing a novel dissolved oxygen (DO) microsensor specially designed for biofilms monitoring. The microsensor was fabricated through standard photolithography techniques, resulting in a microelectrodes array (MEA) of 11 gold circular working electrodes, with a diameter of 50 µm , and a gold reference electrode, which allows obtaining a snapshot oxygen profile of 1 mm of depth. The performance of the sensor was fully characterized under different conditions, inwhich the sensor presented high sensitivity and repeatability, and low detection and quantification limits. Monitoring of sensor performance showed a stable and reliable response. The developed sensor was used in obtaining micropofiles in an aerobic heterotrophic biofilm, showing similar response to Clark-type commercial microsensors. These studies concluded that the novel MEA sensor for DO monitoring allows obtaining oxygen profiles within biofilms, becoming a useful tool for the research of many biological applications.
CitacióGuimera, X. [et al.]. Development of a novel microsensor for the study of oxygen profiles in biofilms. A: International Conference on Biotechniques for Air Pollution Control and Bioenergy. "Biotechniques for air pollution control & bioenergy : biotechniques-2013 : proceedings of the 5th International Conference on Biotechniques for Air Pollution Control and Bioenergy". 2013, p. 275-285.
Dipòsit legal2013
ISBN978-2-35671-058-1
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Guimeraetal-Biotechniques2013.pdf | 751,6Kb | Visualitza/Obre |