Development of a novel microsensor for the study of oxygen profiles in biofilms
Document typeConference lecture
Rights accessOpen Access
Understanding of the processes taking place inside biofilms is a key parameter to progress in the optimization of biofiltration technologies. This study was conducted with the aim of developing a novel dissolved oxygen (DO) microsensor specially designed for biofilms monitoring. The microsensor was fabricated through standard photolithography techniques, resulting in a microelectrodes array (MEA) of 11 gold circular working electrodes, with a diameter of 50 µm , and a gold reference electrode, which allows obtaining a snapshot oxygen profile of 1 mm of depth. The performance of the sensor was fully characterized under different conditions, inwhich the sensor presented high sensitivity and repeatability, and low detection and quantification limits. Monitoring of sensor performance showed a stable and reliable response. The developed sensor was used in obtaining micropofiles in an aerobic heterotrophic biofilm, showing similar response to Clark-type commercial microsensors. These studies concluded that the novel MEA sensor for DO monitoring allows obtaining oxygen profiles within biofilms, becoming a useful tool for the research of many biological applications.
CitationGuimera, X. [et al.]. Development of a novel microsensor for the study of oxygen profiles in biofilms. A: International Conference on Biotechniques for Air Pollution Control and Bioenergy. "Biotechniques for air pollution control & bioenergy : biotechniques-2013 : proceedings of the 5th International Conference on Biotechniques for Air Pollution Control and Bioenergy". 2013, p. 275-285.
All rights reserved. This work is protected by the corresponding intellectual and industrial property rights. Without prejudice to any existing legal exemptions, reproduction, distribution, public communication or transformation of this work are prohibited without permission of the copyright holder