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dc.contributor.authorQi, Yipin
dc.contributor.authorYe, Zhou
dc.contributor.authorFok, Alex
dc.contributor.authorHolmes, Brian N.
dc.contributor.authorEspañol Pons, Montserrat
dc.contributor.authorGinebra Molins, Maria Pau
dc.contributor.authorAparicio Badenas, Conrado José
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.date.accessioned2019-01-11T11:23:33Z
dc.date.issued2018-06-29
dc.identifier.citationQi, Y., Ye, Z., Fok, A., Holmes, B.N., Español, M., Ginebra, M.P., Aparicio, C. Effects of molecular weight and concentration of poly(acrylic acid) on biomimetic mineralization of collagen. "ACS biomaterials science & engineering", 29 Juny 2018, vol. 4, núm. 8, p. 2758-2766.
dc.identifier.issn2373-9878
dc.identifier.urihttp://hdl.handle.net/2117/126583
dc.description.abstractInspired by nature, poly(acrylic acid) (PAA) and other polyelectrolytes have been used as noncollagenous proteins (NCPs) surrogates for biomimetic intrafibrillar mineralization of collagen fibrils and, thus, to model the ultrastructure of bone, to study the mechanism of bone mineralization, and more scarcely to fabricate scaffolds for hard tissue engineering. The objective of this study was to systematically investigate the effect of the molecular weight (MW) and the concentration of PAA on the rate and pattern of biomineralization of collagen matrices. Densified type I collagen films were mineralized in supersaturated PAA-stabilized amorphous calcium-phosphate (PAA-ACP) solutions containing increasing MW (2 kDa, 50 kDa, 450 kDa) and concentrations (10, 25, 50 mg/L) of PAA up to 7 days. The stability and physical properties of collagen-free PAA-ACP solutions were also investigated. In our system, lowering PAA MW and increasing PAA concentration resulted in solutions with increasing stability. Overstable PAA-ACP solutions that fully inhibited mineralization of the collagen matrices were achieved using PAA 2kDa-50mg/L. Conversely, unstable solutions were obtained using high PAA MW at low concentrations. Nucleation and growth of a significant amount of extrafibrillar minerals on the collagen fibrils was obtained using these solutions. In a wide range of combined MW and concentration of PAA, we obtained intrafibrillar mineralization of collagen with hydroxyapatite crystals aligned parallel to the collagen fibril as in natural tissues. Intrafibrillar mineralization was correlated with PAA-ACP stability and growth of the PAA-ACP particles in solution. Our results support using PAA to act as a surrogate to NCPs function as selective inhibitors or promoters of biological mineralization and provide parameters to manufacture new biomimetic scaffolds and constructs for bone and dentin tissue engineering.
dc.format.extent9 p.
dc.language.isoeng
dc.publisherAmerican Chemical Society (ACS)
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshMolecular weights
dc.subject.otheramorphous calcium phosphate
dc.subject.otherintrafibrillar biomineralization
dc.subject.othermolecular weight
dc.subject.otherpoly(acrylic acid)
dc.subject.otherstabilization
dc.titleEffects of molecular weight and concentration of poly(acrylic acid) on biomimetic mineralization of collagen
dc.typeArticle
dc.subject.lemacPesos moleculars
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1021/acsbiomaterials.8b00512
dc.description.peerreviewedPeer Reviewed
dc.rights.accessRestricted access - publisher's policy
drac.iddocument23533823
dc.description.versionPreprint
dc.date.lift10000-01-01
upcommons.citation.authorQi, Y., Ye, Z., Fok, A., Holmes, B.N., Español, M., Ginebra, M.P., Aparicio, C.
upcommons.citation.publishedtrue
upcommons.citation.publicationNameACS biomaterials science & engineering
upcommons.citation.volume4
upcommons.citation.number8
upcommons.citation.startingPage2758
upcommons.citation.endingPage2766


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Except where otherwise noted, content on this work is licensed under a Creative Commons license: Attribution-NonCommercial-NoDerivs 3.0 Spain