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Development of an image sensor platform for microorganisms in water

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Sevilla Dalmau, Daniel
Tutor / directorPruneri, Valerio; Jofre Cruanyes, MarcMés informacióMés informacióMés informació; Martínez, Pedro
Document typeMaster thesis
Date2018-09-06
Rights accessRestricted access - confidentiality agreement
All rights reserved. This work is protected by the corresponding intellectual and industrial property rights. Without prejudice to any existing legal exemptions, reproduction, distribution, public communication or transformation of this work are prohibited without permission of the copyright holder
Abstract
In this thesis, we demonstrate the correct operation of a fluorescence sensor designed for the analysis of water samples, in concrete phytoplankton levels and microorganism counting. The system, based on different techniques of flow cytometry and fluorometry, makes use of a laser source as well as an objective microscope focused on a capillary, without need for a cuvette, to interact with the sample. Measurements on cell counting and phytoplankton photochemical efficiency were achieved for different sized cells. An overview of the different modules of the system is given as well as the final results obtained.
 
En aquesta tesis demostrem el correcte funcionament d'un sensor de fluorescència disenyat específicament per l'anàlisis de mostres d'aigua, en concret, els seus nivells de fitoplancton i microorganismes. El sistema, basat en diferents tècniques de citometria de fluxe i fluorometria, consta d'un laser juntament amb un microscopi enfocat en un capilar, sense necessitat d'una cuveta, per interaccionar amb la mostra. Mesures de conteig de microorganismes i de la vitalitat dels nivells de plancton van ser obtinguts per cèl·lules de diferents tamanys. Un resum dels diferents mòduls del sistema i els resultats finals obtinguts són mostrats.
Description
The project will focus on developing an optical platform using CMOS image sensors to detect micro-organisms in drinking, industrial and sea waters
SubjectsLasers, Electron optics, Biosensors, Oceanography, Làsers, Òptica electrònica, Biosensors, Oceanografia
URIhttp://hdl.handle.net/2117/124619
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  • Màsters oficials - Master's degree in Photonics [298]
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