Enzymatic synthesis of a thiolated chitosan-based wound dressing crosslinked with chicoric acid
PublisherRoyal Society of Chemistry (RSC)
Rights accessOpen Access
This work describes the enzymatic synthesis of multifunctional hydrogels for chronic wound treatment using thiolated chitosan and the natural polyphenol chicoric acid. Gelation was achieved by laccase-catalyzed oxidation of chicoric acid, a natural compound used for the first time as a homobifunctional crosslinker, reacting subsequently with nucleophilic thiol and amino groups from the chitosan derivative. This approach allowed for twice-faster gelation at three-fold reduced crosslinking reagent concentration, compared to reported enzymatic synthesis of hydrogels using gallic acid as a phenolic provider. Hydrogels with 600 % swelling capacity, coupled to only 20 % weight loss after 6 days in physiological conditions, were obtained. The clinically relevant gram-positive Staphylococcus aureus and the gram-negative Pseudomonas aeruginosa, were reduced by up to 4.5 and 5.5 logs, respectively. A tunable, in the range of 20 - 95 %, ex-vivo inhibition of myeloperoxidase (MPO) activity in chronic wound exudate was achieved, together with control over the total matrix metalloproteinases (MMPs) activities.
CitationStefanov, I., Hinojosa, D., Maspoch, S., Hoyo, J., Tzanov, T. Enzymatic synthesis of a thiolated chitosan-based wound dressing crosslinked with chicoric acid. "Journal of materials chemistry B", 26 Octubre 2018, vol.6, p. 7943-7953