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Biomimetic engineering of conductive curli protein films
dc.contributor.author | Dorval Courchesne, Noémie-Manuelle |
dc.contributor.author | DeBenedictis, Elizabeth P. |
dc.contributor.author | Tresback, Jason |
dc.contributor.author | Zanuy Gomara, David |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament d'Enginyeria Química |
dc.date.accessioned | 2018-10-19T10:01:40Z |
dc.date.available | 2018-10-19T10:01:40Z |
dc.date.issued | 2018-09-11 |
dc.identifier.citation | Dorval, N.-M., DeBenedictis, E., Tresback, J., Zanuy, D. Biomimetic engineering of conductive curli protein films. "Nanotechnology", 11 Setembre 2018, vol. 29, núm. 45, p. 454002-454013. |
dc.identifier.issn | 0957-4484 |
dc.identifier.uri | http://hdl.handle.net/2117/122652 |
dc.description.abstract | Bioelectronic systems derived from peptides and proteins are of particular interest for fabricating novel flexible, biocompatible and bioactive devices. These synthetic or recombinant systems designed for mediating electron transport often mimic the proteinaceous appendages of naturally occurring electroactive bacteria. Drawing inspiration from such conductive proteins with a high content of aromatic residues, we have engineered a fibrous protein scaffold, curli fibers produced by Escherichia coli bacteria, to enable long-range electron transport. We report the genetic engineering and characterization of curli fibers containing aromatic residues of different nature, with defined spatial positioning, and with varying content on single self-assembling CsgA curli subunits. Our results demonstrate the impressive versatility of the CsgA protein for genetically engineering protein-based materials with new functions. Through a scalable purification process, we show that macroscopic gels and films can be produced, with engineered thin films exhibiting a greater conductivity compared with wild-type curli films. We anticipate that this engineered conductive scaffold, and our approach that combines computational modeling, protein engineering, and biosynthetic manufacture will contribute to the improvement of a range of useful bio-hybrid technologies. |
dc.format.extent | 12 p. |
dc.language.iso | eng |
dc.subject | Àrees temàtiques de la UPC::Enginyeria química |
dc.subject.lcsh | Amino acids |
dc.subject.lcsh | Proteins |
dc.subject.other | protein materials |
dc.subject.other | bio-conductivity |
dc.subject.other | amyloids |
dc.subject.other | aromatic amino acids |
dc.title | Biomimetic engineering of conductive curli protein films |
dc.type | Article |
dc.subject.lemac | Aminoàcids |
dc.contributor.group | Universitat Politècnica de Catalunya. IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies |
dc.identifier.doi | 10.1088/1361-6528/aadd3a |
dc.description.peerreviewed | Peer Reviewed |
dc.relation.publisherversion | http://iopscience.iop.org/article/10.1088/1361-6528/aadd3a |
dc.rights.access | Open Access |
local.identifier.drac | 23390872 |
dc.description.version | Preprint |
local.citation.author | Dorval, N.-M.; DeBenedictis, E.; Tresback, J.; Zanuy, D. |
local.citation.publicationName | Nanotechnology |
local.citation.volume | 29 |
local.citation.number | 45 |
local.citation.startingPage | 454002 |
local.citation.endingPage | 454013 |
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