Two different strategies to enhance osseointegration in porous titanium: Inorganic thermo-chemical treatment versus organic coating by peptide adsorption
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In this study, highly-interconnected porous titanium implants were produced by powder sintering with different porous diameters and open interconnectivity. The actual foams were produced using high cost technologies: Chemical Vapor Deposition (CVD), Physical Vapor Deposition (PVD), and spark plasma sintering, and the porosity and/or interconnection was not optimized. The aim was to generate a bioactive surface on foams using two different strategies, based on inorganic thermo-chemical treatment and organic coating by peptide adsorption, to enhance osseointegration. Porosity was produced using NaCl as a space holder and polyethyleneglicol as a binder phase. Static and fatigue tests were performed in order to determine mechanical behaviors. Surface bioactivation was performed using a thermo-chemical treatment or by chemical adsorption with peptides. Osteoblast-like cells were cultured and cytotoxicity was measured. Bioactivated scaffolds and a control were implanted in the tibiae of rabbits. Histomorphometric evaluation was performed at 4 weeks after implantation. Interconnected porosity was 53% with an average diameter of 210 µm and an elastic modulus of around 1 GPa with good mechanical properties. The samples presented cell survival values close to 100% of viability. Newly formed bone was observed inside macropores, through interconnected porosity, and on the implant surface. Successful bone colonization of inner structure (40%) suggested good osteoconductive capability of the implant. Bioactivated foams showed better results than non-treated ones, suggesting both bioactivation strategies induce osteointegration capability.
CitationOrtiz-Hernández, M., Rappe, K., Molmeneu, M., Mas-Moruno, C., Guillem-Marti, J., Punset, M., Caparrós, C., Calero, J., Franch, J., Fernández-Fairén, M., Gil, J. Two different strategies to enhance osseointegration in porous titanium: Inorganic thermo-chemical treatment versus organic coating by peptide adsorption. "International journal of molecular sciences", 30 Agost 2018, vol. 19, núm. 9, p. 2574-1-2574-17.