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dc.contributor.authorMorales Larramendi, Ricardo
dc.contributor.authorRiss, M
dc.contributor.authorWang, Liang
dc.contributor.authorGavin, R
dc.contributor.authorDel, Rio Ja
dc.contributor.authorAlcubilla González, Ramón
dc.contributor.authorClaverol Tinturé, Enrique
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica
dc.date.accessioned2018-09-21T18:53:49Z
dc.date.issued2008-11
dc.identifier.citationMorales, R., Riss, M., Liang, W., Gavin, R., Del, R., Alcubilla, R., Claverol, E. Integrating multi-unit electrophysiology and plastic culture dishes for network neuroscience. "Lab on a chip", Novembre 2008, vol. 8, núm. 11, p. 1896-1905.
dc.identifier.issn1473-0197
dc.identifier.urihttp://hdl.handle.net/2117/121416
dc.description.abstractThe electrophysiological characterisation of cultured neurons is of paramount importance for drug discovery, safety pharmacology and basic research in the neurosciences. Technologies offering low cost, low technical complexity and potential for scalability towards high-throughput electrophysiology on in vitro neurons would be advantageous, in particular for screening purposes. Here we describe a plastic culture substrate supporting low-complexity multi-unit loose-patch recording and stimulation of developing networks while retaining manufacturability compatible with low-cost and large-scale production. Our hybrid polydimethylsilane (PDMS)-on-polystyrene structures include chambers (6 mm in diameter) and microchannels (25 microm x 3.7 microm x 1 mm) serving as substrate-embedded recording pipettes. Somas are plated and retained in the chambers due to geometrical constraints and their processes grow along the microchannels, effectively establishing a loose-patch configuration without human intervention. We demonstrate that off-the-shelf voltage-clamp, current-clamp and extracellular amplifiers can be used to record and stimulate multi-unit activity with the aid of our dishes. Spikes up to 50 pA in voltage-clamp and 300 microV in current-clamp modes are recorded in sparse and bursting activity patterns characteristic of 1 week-old hippocampal cultures. Moreover, spike sorting employing principal component analysis (PCA) confirms that single microchannels support the recording of multiple neurons. Overall, this work suggests a strategy to endow conventional culture plasticware with added functionality to enable cost-efficient network electrophysiology.
dc.format.extent10 p.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Ciències de la salut::Medicina::Neurologia
dc.subjectÀrees temàtiques de la UPC::Ciències de la salut::Medicina::Anatomia i fisiologia humana
dc.subject.lcshNeurosciences
dc.subject.lcshElectrophysiology
dc.titleIntegrating multi-unit electrophysiology and plastic culture dishes for network neuroscience
dc.typeArticle
dc.subject.lemacNeurociències
dc.subject.lemacElectrofisiologia
dc.contributor.groupUniversitat Politècnica de Catalunya. MNT - Grup de Recerca en Micro i Nanotecnologies
dc.identifier.doi10.1039/B802165A
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://pubs.rsc.org/en/Content/ArticleLanding/2008/LC/b802165a#!divAbstract
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac764050
dc.description.versionPostprint (published version)
dc.date.lift10000-01-01
local.citation.authorMorales, R.; Riss, M.; Liang, W.; Gavin, R.; Del, R.; Alcubilla, R.; Claverol, E.
local.citation.publicationNameLab on a chip
local.citation.volume8
local.citation.number11
local.citation.startingPage1896
local.citation.endingPage1905


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