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dc.contributor.authorPortell Canal, Xavier
dc.contributor.authorGinovart Gisbert, Marta
dc.contributor.authorCarbó Moliner, Rosa
dc.contributor.authorGras Moreu, Anna Maria
dc.contributor.authorVives Rego, Josep
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Matemàtica Aplicada III
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia
dc.identifier.citationPortell, X. [et al.]. Population analysis of a commercial Saccharomyces cerevisiae wine yeast in a batch culture by electric particle analysis, light difraction and flow cytometry. "Fems yeast research", 2010, vol. 11, p. 18-28.
dc.description.abstractData from electric particle analysis, light diffraction and flow cytometry analysis provide information on changes in cell morphology. Here, we report analyses of Saccharomyces cerevisiae populations growing in a batch culture using these techniques. The size distributions were determined by electric particle analysis and by light diffraction in order to compare their outcomes. Flow cytometry parameters forward (related to cell size) and side (related to cell granularity) scatter were also determined to complement this information. These distributions of yeast properties were analysed statistically and by a complexity index. The cell size of Saccharomyces at the lag phase was smaller than that at the beginning of the exponential phase, whereas during the stationary phase, the cell size converged with the values observed during the lag phase. These experimental techniques, when used together, allow us to distinguish among and characterize the cell size, cell granularity and the structure of the yeast population through the three growth phases. Flow cytometry patterns are better than light diffraction and electric particle analysis in showing the existence of subpopulations during the different phases, especially during the stationary phase. The use of a complexity index in this context helped to differentiate these phases and confirmed the yeast cell heterogeneity.
dc.format.extent11 p.
dc.subjectÀrees temàtiques de la UPC::Enginyeria agroalimentària::Indústries agroalimentàries::Biotecnologia alimentària
dc.subject.lcshFlow cytometry
dc.subject.otheryeast populations cell size distributions particle size analysis light diffraction flow cytometry batch culture
dc.titlePopulation analysis of a commercial Saccharomyces cerevisiae wine yeast in a batch culture by electric particle analysis, light difraction and flow cytometry
dc.subject.lemacBiotecnologia -- Indústria i comerç
dc.contributor.groupUniversitat Politècnica de Catalunya. SC-SIMBIO - Sistemes complexos. Simulació discreta de materials i de sistemes biològics
dc.rights.accessRestricted access - publisher's policy
dc.description.versionPostprint (published version)
upcommons.citation.authorPortell, X.; Ginovart, M.; Carbó, R.; Gras, A.; Vives, J.
upcommons.citation.publicationNameFems yeast research

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